Anderson K D, Reiner A
Department of Anatomy and Neurobiology, University of Tennessee, Memphis 38163.
Brain Res. 1991 Dec 24;568(1-2):235-43. doi: 10.1016/0006-8993(91)91403-n.
Immunohistochemical double-label techniques were used to study the localization of DARPP-32, a phosphoprotein that is enriched in neurons possessing members of the D1 subfamily of dopamine receptors, in several different types of striatal neurons in the rat basal ganglia. The vast majority (94.1%) of striatonigral projection neurons (the vast majority of which contain substance P), identified by retrograde labeling with fluorogold, were observed to contain DARPP-32. Similarly, the vast majority of striatopallidal projection neurons (87.7%), identified by immunofluorescence labeling for enkephalin (ENK), were found to label for DARPP-32. In contrast, cholinergic and neuropeptide Y-containing striatal interneurons were never observed to contain DARPP-32. These results suggest that essentially all major types of striatal medium spiny projection neurons may possess members of the D1 subfamily of dopamine receptors, but that striatal local circuit neurons do not possess members of the D1 subfamily of receptors.
免疫组织化学双标记技术被用于研究DARPP - 32（一种富含于拥有多巴胺受体D1亚家族成员的神经元中的磷蛋白）在大鼠基底神经节几种不同类型纹状体神经元中的定位。通过用荧光金逆行标记鉴定出的绝大多数（94.1%）纹状体黑质投射神经元（其中绝大多数含有P物质）被观察到含有DARPP - 32。同样，通过脑啡肽（ENK）免疫荧光标记鉴定出的绝大多数纹状体苍白球投射神经元（87.7%）被发现标记有DARPP - 32。相比之下，从未观察到胆碱能和含神经肽Y的纹状体中间神经元含有DARPP - 32。这些结果表明，基本上所有主要类型的纹状体中等棘状投射神经元可能拥有多巴胺受体D1亚家族的成员，但纹状体局部回路神经元不拥有受体D1亚家族的成员。
