Kaluzhny Dmitry N, Beniaminov Artemy D, Minyat Elvira E
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova, 32, Moscow, 199911, Russia.
J Biomol Struct Dyn. 2008 Jun;25(6):663-7. doi: 10.1080/07391102.2008.10507213.
The fluorescent 2-aminopurine probe (2-AP) incorporated into the loop of 23-mer RNA hairpin of HIV-1 genome dimerization initiation site (DIS) was used for discrimination of specific and unspecific binding of paromomycin and spermine to the kissing loop dimer (KD) formed in solution. While both ligands stabilized the KD RNA structure, only paromomycin binding resulted in significant increase of 2-AP fluorescence. These observations suggest that the 2-AP fluorescent RNA construct might be useful for selecting ligands specifically binding the HIV-1 kissing loop RNA dimer.
将荧光2-氨基嘌呤探针（2-AP）掺入HIV-1基因组二聚化起始位点（DIS）的23聚体RNA发夹环中，用于区分巴龙霉素和精胺与溶液中形成的亲吻环二聚体（KD）的特异性和非特异性结合。虽然两种配体都稳定了KD RNA结构，但只有巴龙霉素结合导致2-AP荧光显著增加。这些观察结果表明，2-AP荧光RNA构建体可能有助于筛选特异性结合HIV-1亲吻环RNA二聚体的配体。
