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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Kim S Z, Cho K W, Kim S H, Ryu H, Hwang Y H, Lee G Y

Department of Physiology, Medical School, Jeonbug National University, Republic of Korea.

Comp Biochem Physiol B. 1991;100(3):575-8. doi: 10.1016/0305-0491(91)90223-z.

The presence and partial characterization of immunoreactive atrial natriuretic peptide (ir-ANP) in the bulbus arteriosus were investigated using high performance liquid chromatography (HPLC) and radioimmunoassay (RIA) in several freshwater teleostean fish (catfish, silver crusian carp, snakehead, and rice eel). 2. The level of ir-ANP in the bulbus arteriosus was comparable with that in the atria at about 5-15 pg/mg, 6-13 pg/mg wet wt, respectively, but was 2-4-fold higher than that in the ventricles in all species observed. 3. The HPLC profile of ir-ANP showed two main peaks corresponding to low and high mol. wt rat ANPs. 4. Our results provide evidence for the presence of ir-ANP with high as well as low mol. wts in the bulbus arteriosus of freshwater teleostean fish.

使用高效液相色谱法（HPLC）和放射免疫分析法（RIA），对几种淡水硬骨鱼（鲶鱼、银鲫、乌鳢和黄鳝）动脉球中免疫反应性心房利钠肽（ir-ANP）的存在情况及其部分特性进行了研究。2. 动脉球中ir-ANP的水平分别约为5-15 pg/mg、6-13 pg/mg湿重，与心房中的水平相当，但在所观察的所有物种中，均比心室中的水平高2-4倍。3. ir-ANP的HPLC图谱显示出两个主要峰，分别对应低分子量和高分子量的大鼠ANP。4. 我们的结果为淡水硬骨鱼动脉球中存在高分子量和低分子量的ir-ANP提供了证据。

Kim S Z, Cho K W, Kim S H, Ryu H, Hwang Y H, Lee G Y

Department of Physiology, Medical School, Jeonbug National University, Republic of Korea.

Comp Biochem Physiol B. 1991;100(3):575-8. doi: 10.1016/0305-0491(91)90223-z.

The presence and partial characterization of immunoreactive atrial natriuretic peptide (ir-ANP) in the bulbus arteriosus were investigated using high performance liquid chromatography (HPLC) and radioimmunoassay (RIA) in several freshwater teleostean fish (catfish, silver crusian carp, snakehead, and rice eel). 2. The level of ir-ANP in the bulbus arteriosus was comparable with that in the atria at about 5-15 pg/mg, 6-13 pg/mg wet wt, respectively, but was 2-4-fold higher than that in the ventricles in all species observed. 3. The HPLC profile of ir-ANP showed two main peaks corresponding to low and high mol. wt rat ANPs. 4. Our results provide evidence for the presence of ir-ANP with high as well as low mol. wts in the bulbus arteriosus of freshwater teleostean fish.

使用高效液相色谱法（HPLC）和放射免疫分析法（RIA），对几种淡水硬骨鱼（鲶鱼、银鲫、乌鳢和黄鳝）动脉球中免疫反应性心房利钠肽（ir-ANP）的存在情况及其部分特性进行了研究。2. 动脉球中ir-ANP的水平分别约为5-15 pg/mg、6-13 pg/mg湿重，与心房中的水平相当，但在所观察的所有物种中，均比心室中的水平高2-4倍。3. ir-ANP的HPLC图谱显示出两个主要峰，分别对应低分子量和高分子量的大鼠ANP。4. 我们的结果为淡水硬骨鱼动脉球中存在高分子量和低分子量的ir-ANP提供了证据。