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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Kim S H, Cho K W, Oh S H, Hwang Y H, Lim S H, Ryu H, Seul K H, Jeong G B, Yoon S

Department of Physiology, Jeonbug National University Medical School, Jeonju, Republic of Korea.

Comp Biochem Physiol Comp Physiol. 1993 Feb;104(2):219-23. doi: 10.1016/0300-9629(93)90306-o.

The presence and partial characterization of immunoreactive ANP (irANP) in eggs were investigated using high performance liquid chromatography (HPLC), immunohistochemistry and Northern-blot hybridization in mammalian and nonmammalian vertebrates. 2. Serial dilution curves of egg extracts from frogs and freshwater teleostean fishes (silver crusian carp and loach) were parallel to the standard curve of atriopeptin III. Rat oocytes also contained irANP (25-30 pg/egg). 3. The HPLC profile of irANP showed two main peaks corresponding to low and high molecular weight of irANP. 4. ANP mRNA was detected in the fish eggs. With immunohistochemical analysis of the rat ovarian follicles, this ANP-like material was localized mainly at the oocytes. 5. We, therefore, suggest that the vertebrate oocytes synthesize ANP and that the presence of ANP in the oocytes may be linked with the cell differentiation.

运用高效液相色谱法（HPLC）、免疫组织化学和Northern印迹杂交技术，对哺乳动物和非哺乳动物脊椎动物卵中免疫反应性心房钠尿肽（irANP）的存在情况及其部分特性进行了研究。2. 青蛙和淡水硬骨鱼类（银鲫和泥鳅）卵提取物的系列稀释曲线与心房肽III的标准曲线平行。大鼠卵母细胞也含有irANP（25 - 30皮克/卵）。3. irANP的HPLC图谱显示出两个主要峰，分别对应于低分子量和高分子量的irANP。4. 在鱼卵中检测到了ANP mRNA。通过对大鼠卵巢卵泡的免疫组织化学分析，这种类似ANP的物质主要定位于卵母细胞。5. 因此，我们认为脊椎动物卵母细胞能够合成ANP，并且卵母细胞中ANP的存在可能与细胞分化有关。

Kim S H, Cho K W, Oh S H, Hwang Y H, Lim S H, Ryu H, Seul K H, Jeong G B, Yoon S

Department of Physiology, Jeonbug National University Medical School, Jeonju, Republic of Korea.

Comp Biochem Physiol Comp Physiol. 1993 Feb;104(2):219-23. doi: 10.1016/0300-9629(93)90306-o.

The presence and partial characterization of immunoreactive ANP (irANP) in eggs were investigated using high performance liquid chromatography (HPLC), immunohistochemistry and Northern-blot hybridization in mammalian and nonmammalian vertebrates. 2. Serial dilution curves of egg extracts from frogs and freshwater teleostean fishes (silver crusian carp and loach) were parallel to the standard curve of atriopeptin III. Rat oocytes also contained irANP (25-30 pg/egg). 3. The HPLC profile of irANP showed two main peaks corresponding to low and high molecular weight of irANP. 4. ANP mRNA was detected in the fish eggs. With immunohistochemical analysis of the rat ovarian follicles, this ANP-like material was localized mainly at the oocytes. 5. We, therefore, suggest that the vertebrate oocytes synthesize ANP and that the presence of ANP in the oocytes may be linked with the cell differentiation.

运用高效液相色谱法（HPLC）、免疫组织化学和Northern印迹杂交技术，对哺乳动物和非哺乳动物脊椎动物卵中免疫反应性心房钠尿肽（irANP）的存在情况及其部分特性进行了研究。2. 青蛙和淡水硬骨鱼类（银鲫和泥鳅）卵提取物的系列稀释曲线与心房肽III的标准曲线平行。大鼠卵母细胞也含有irANP（25 - 30皮克/卵）。3. irANP的HPLC图谱显示出两个主要峰，分别对应于低分子量和高分子量的irANP。4. 在鱼卵中检测到了ANP mRNA。通过对大鼠卵巢卵泡的免疫组织化学分析，这种类似ANP的物质主要定位于卵母细胞。5. 因此，我们认为脊椎动物卵母细胞能够合成ANP，并且卵母细胞中ANP的存在可能与细胞分化有关。