Ferrario A, Rucker N, Ryter S W, Doiron D R, Gomer C J
Clayton Ocular Oncology Center, Childrens Hospital of Los Angeles, CA 90027.
Lasers Surg Med. 1991;11(5):404-10. doi: 10.1002/lsm.1900110504.
A pulsed KTP pumped dye laser (25 kHz repetition rate and 470 nsec pulse width) has been compared to a continuous wave argon ion pumped dye laser as the source of 630 nm light during in-vitro and in-vivo Photofrin-II mediated photosensitization studies. Individual experiments documented the effectiveness of each laser system on a) photosensitizer induced cytotoxicity and induction of stress protein synthesis using Chinese hamster fibroblasts; b) photobleaching of Photofrin-II in aqueous solution; c) Photofrin II mediated photosensitization of normal mouse skin; d) Photofrin II mediated photodynamic therapy of a mouse mammary carcinoma; and e) tumor temperature levels generated during laser exposure. Comparable results were obtained for both laser systems in all experiments.
在体外和体内的卟吩姆钠介导的光致敏研究中，已将一台脉冲式KTP泵浦染料激光器（重复频率25kHz，脉冲宽度470纳秒）与一台连续波氩离子泵浦染料激光器作为630nm光源进行了比较。各项实验记录了每个激光系统在以下方面的有效性：a）使用中国仓鼠成纤维细胞进行的光敏剂诱导的细胞毒性和应激蛋白合成诱导；b）水溶液中卟吩姆钠的光漂白；c）正常小鼠皮肤的卟吩姆钠介导的光致敏；d）小鼠乳腺癌的卟吩姆钠介导的光动力疗法；以及e）激光照射期间产生的肿瘤温度水平。在所有实验中，两种激光系统均获得了可比的结果。
