Rodríguez-Miranda Esmeralda, Buffone Mariano G, Edwards Scott E, Ord Teri S, Lin Kathleen, Sammel Mary D, Gerton George L, Moss Stuart B, Williams Carmen J
Department of Obstetrics & Gynecology, Center for Research on Reproduction & Women's Health, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Biol Reprod. 2008 Jul;79(1):164-71. doi: 10.1095/biolreprod.107.065565. Epub 2008 Apr 9.
Extracellular adenosine 5'-triphosphate (ATPe) treatment of human sperm has been implicated in improving in vitro fertilization (IVF) results. We used the mouse model to investigate mechanisms of action of ATPe on sperm. ATPe treatment significantly enhanced IVF success as indicated by both rate of pronuclear formation and percentage cleavage to the 2-cell stage. However, ATPe did not increase the percentage of sperm undergoing spontaneous acrosomal exocytosis nor change the pattern of protein tyrosine phosphorylation normally observed in capacitated sperm. ATPe altered sperm motility parameters; in particular, both noncapacitated and capacitated sperm swam faster and straighter. The percentage of hyperactivated sperm did not increase in capacitated ATPe-treated sperm compared to control sperm. ATPe induced a rapid increase in the level of intracellular calcium that was inhibited by two distinct P2 purinergic receptor inhibitors, confirming that these receptors have an ionotropic role in sperm function. The observed motility changes likely explain, in part, the improved fertilizing capability when ATPe-treated sperm were used in IVF procedures and suggest a mechanism by which ATPe treatment may be beneficial for artificial reproductive techniques.
细胞外5'-三磷酸腺苷（ATPe）处理人类精子与改善体外受精（IVF）结果有关。我们使用小鼠模型来研究ATPe对精子的作用机制。如原核形成率和2细胞期分裂百分比所示，ATPe处理显著提高了IVF成功率。然而，ATPe并没有增加自发顶体胞吐的精子百分比，也没有改变在获能精子中通常观察到的蛋白质酪氨酸磷酸化模式。ATPe改变了精子运动参数；特别是，未获能和获能的精子游动得更快、更直。与对照精子相比，获能的ATPe处理精子中高活性精子的百分比没有增加。ATPe诱导细胞内钙水平迅速升高，这被两种不同的P2嘌呤能受体抑制剂所抑制，证实这些受体在精子功能中具有离子otropic作用。观察到的运动变化可能部分解释了在IVF程序中使用ATPe处理的精子时受精能力的提高，并提示了ATPe处理可能对辅助生殖技术有益的一种机制。
