Meade J C, Stringer J R
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati Medical Center, Ohio 45267-0524.
J Protozool. 1991 Nov-Dec;38(6):66S-68S.
Oligonucleotide primers were used to amplify DNA sequences from a plasma membrane cation transporting ATPase gene and a transcription factor IID (TFIID) gene from Pneumocystis carinii genomic DNA. The entire P. carinii ATPase gene was cloned from a genomic library by hybridization to the PCR-amplified DNA product. The nucleotide sequence of the gene contained a 2,799 base-pair open reading frame that encoded a 102,274 dalton protein composed of 933 amino acids. The P. carinii ATPase protein was 69-74% identical to four fungal proton pumps but less than 35% identical to protozoan and mammalian cation transporting ATPase genes or the Ca++ ATPases of Saccharomyces. The nucleotide sequence of a portion of the TFIID gene could be translated to produce a peptide of 53 amino acids in two regions of the sequence, interrupted by a 45 bp intron. The predicted TFIID amino acid sequence was identical to yeast TFIID genes in this region.
使用寡核苷酸引物从卡氏肺孢子虫基因组DNA中扩增质膜阳离子转运ATP酶基因和转录因子IID（TFIID）基因的DNA序列。通过与PCR扩增的DNA产物杂交，从基因组文库中克隆出完整的卡氏肺孢子虫ATP酶基因。该基因的核苷酸序列包含一个2799个碱基对的开放阅读框，编码一个由933个氨基酸组成、分子量为102274道尔顿的蛋白质。卡氏肺孢子虫ATP酶蛋白与四种真菌质子泵的同源性为69 - 74%，但与原生动物和哺乳动物阳离子转运ATP酶基因或酿酒酵母的Ca++ ATP酶的同源性低于35%。TFIID基因部分核苷酸序列在两个区域可翻译产生一个53个氨基酸的肽段，中间被一个45 bp的内含子打断。预测的TFIID氨基酸序列在该区域与酵母TFIID基因相同。
