National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, Japan.
Theor Appl Genet. 2013 Sep;126(9):2417-25. doi: 10.1007/s00122-013-2145-2.
Bacterial seedling rot (BSR), a destructive disease of rice (Oryza sativa L.), is caused by the bacterial pathogen Burkholderia glumae. To identify QTLs for resistance to BSR, we conducted a QTL analysis using chromosome segment substitution lines (CSSLs) derived from a cross between Nona Bokra (resistant) and Koshihikari (susceptible). Comparison of the levels of BSR in the CSSLs and their recurrent parent, Koshihikari, revealed that a region on chromosome 10 was associated with resistance. Further genetic analyses using an F5 population derived from a cross between a resistant CSSL and Koshihikari confirmed that a QTL for BSR resistance was located on the short arm of chromosome 10. The Nona Bokra allele was associated with resistance to BSR. Substitution mapping in the Koshihikari genetic background demonstrated that the QTL, here designated as qRBS1 (quantitative trait locus for RESISTANCE TO BACTERIAL SEEDLING ROT 1), was located in a 393-kb interval (based on the Nipponbare reference genome sequence) defined by simple sequence repeat markers RM24930 and RM24944.
细菌性基腐病（BSR）是一种严重危害水稻（Oryza sativa L.）的细菌性病害，由细菌病原体稻生欧文氏菌（Burkholderia glumae）引起。为了鉴定抗 BSR 的 QTL，我们利用 Nona Bokra（抗性）和 Koshihikari（感病）杂交产生的染色体片段代换系（CSSLs）进行了 QTL 分析。比较 CSSLs 及其轮回亲本 Koshihikari 中的 BSR 水平，发现第 10 号染色体上的一个区域与抗性有关。进一步利用一个来源于抗性 CSSL 和 Koshihikari 杂交的 F5 群体进行遗传分析，证实了一个抗 BSR 的 QTL 位于第 10 号染色体的短臂上。Nona Bokra 等位基因与抗 BSR 有关。在 Koshihikari 遗传背景下的替换作图表明，该 QTL 被命名为 qRBS1（对细菌性基腐病的抗性的数量性状位点 1），位于简单重复序列标记 RM24930 和 RM24944 定义的 393-kb 区间内（基于 Nipponbare 参考基因组序列）。
