Haffajee A D, Socransky S S, Patel M R, Song X
Department of Periodontology, The Forsyth Institute, Boston, MA 02115, USA.
Oral Microbiol Immunol. 2008 Jun;23(3):196-205. doi: 10.1111/j.1399-302X.2007.00411.x.
BACKGROUND/AIMS: To examine microbial communities in supragingival biofilm samples.
Supragingival plaque samples were taken from 187 subjects at baseline (n = 4745). Fifty-five subjects provided supragingival plaque samples at 1-7 days after professional tooth cleaning (n = 1456); 93 subjects provided 8044 samples between 3 and 24 months post-therapy. All samples were individually analyzed for their content of 40 bacterial species using checkerboard DNA-DNA hybridization. Microbial associations among species were sought using cluster analysis and community ordination techniques for the three groups separately.
Six complexes were formed for the baseline samples. Similar complexes were formed for the samples taken 3-24 months post-therapy. However, distinct changes were observed in microbial communities in samples taken during the 7 days of plaque redevelopment. The complexes related to clinical parameters of periodontal disease.
There were specific microbial complexes in supragingival plaque that were similar to those found in subgingival plaque samples with a few minor differences. The relation of previously unclustered taxa to the complexes was also described.
背景/目的：检测龈上生物膜样本中的微生物群落。
在基线时从187名受试者采集龈上菌斑样本（n = 4745）。55名受试者在专业洗牙后1 - 7天提供龈上菌斑样本（n = 1456）；93名受试者在治疗后3至24个月提供了8044份样本。使用棋盘DNA - DNA杂交技术对所有样本分别分析40种细菌的含量。分别对三组样本使用聚类分析和群落排序技术寻找物种间的微生物关联。
基线样本形成了六个复合体。治疗后3 - 24个月采集的样本形成了类似的复合体。然而，在菌斑再形成的7天内采集的样本中观察到微生物群落有明显变化。这些复合体与牙周病的临床参数相关。
龈上菌斑中存在特定的微生物复合体，与龈下菌斑样本中发现的复合体相似，略有差异。还描述了先前未聚类的分类群与复合体的关系。
