Samija Ivan, Matesa Neven, Lukac Josip, Kusic Zvonko
Department of Oncology and Nuclear Medicine, University Hospital "Sestre milosrdnice," Zagreb, Croatia.
Cancer. 2008 Jun 25;114(3):187-95. doi: 10.1002/cncr.23498.
Analysis of different tumor markers by reverse transcriptase-polymerase chain reaction (RT-PCR) in fine-needle aspiration samples of thyroid nodules has been studied with the objective of improving the accuracy of the preoperative diagnosis of thyroid lesions. The aim of the current study was to investigate thyroid fine-needle aspiration samples inadequate for RT-PCR analysis and to determine whether there is a correlation between their proportion and the method of sampling used or the greatest dimension of the nodules.
A total of 350 fine-needle aspiration samples from patients with thyroid nodules were analyzed. After the aspirate was smeared for conventional cytology, the leftover material in the needle was used for RT-PCR analysis in 1 group of 175 patients. In another group of 175 patients, a separate puncture was performed to obtain material for RT-PCR analysis only. Samples were considered adequate for RT-PCR analysis if the expression of both glyceraldehyde-3-phosphate dehydrogenase and thyroglobulin was found by RT-PCR.
In total, 61 (17.4%) samples inadequate for RT-PCR were detected. All 12 samples that were inadequate for cytologic diagnosis were also found to be inadequate for RT-PCR analysis. The proportion of inadequate samples for RT-PCR was found to be significantly higher in samples taken from leftover material in the needle (21.7%) then in samples from a separate puncture (13.1%) (P = .049). No statistically significant correlation between the adequacy of samples for RT-PCR and the largest dimension of the nodule was found.
The proportion of samples inadequate for RT-PCR was found to be higher in samples taken from leftover material in the needle than in samples obtained from a separate puncture.
通过逆转录聚合酶链反应（RT-PCR）分析甲状腺结节细针穿刺样本中的不同肿瘤标志物，旨在提高甲状腺病变术前诊断的准确性。本研究的目的是调查不适用于RT-PCR分析的甲状腺细针穿刺样本，并确定其比例与所采用的采样方法或结节的最大直径之间是否存在相关性。
共分析了350例甲状腺结节患者的细针穿刺样本。将抽吸物涂片进行常规细胞学检查后，在一组175例患者中，针内剩余材料用于RT-PCR分析。在另一组175例患者中，单独进行穿刺以仅获取用于RT-PCR分析的材料。如果通过RT-PCR检测到甘油醛-3-磷酸脱氢酶和甲状腺球蛋白的表达，则认为样本适用于RT-PCR分析。
总共检测到61例（17.4%）不适用于RT-PCR的样本。所有12例细胞学诊断不充分的样本也被发现不适用于RT-PCR分析。发现从针内剩余材料获取的样本中不适用于RT-PCR的样本比例（21.7%）显著高于单独穿刺获取的样本（13.1%）（P = 0.049）。未发现样本适用于RT-PCR与结节最大直径之间存在统计学显著相关性。
发现从针内剩余材料获取的样本中不适用于RT-PCR的样本比例高于从单独穿刺获取的样本。
