在猪细胞系IB-RS-2培养物中检测牛干扰素。
Assay of bovine interferons in cultures of the porcine cell line IB-RS-2.
作者信息
Ahl R, Rump A
出版信息
Infect Immun. 1976 Sep;14(3):603-6. doi: 10.1128/iai.14.3.603-606.1976.
Abstract
An assay for bovine interferons has been developed using the porcine cell line IB-RS-2 and a bovine enterovirus, CBV-D, as challenge virus. The method is based on estimation of cytopathic effect measured by uptake of neutral red. Teh assay is simple, sensitive, and reproducible. A comparative test of different viruses in IB-RS-2 cells and secondary calf kidney cells revealed that the sensitivity of a virus to interferon can vary up to 1,000-fold in the two cell systems. Vesicular stomatitis virus was found to be rather insensitive to interferon in IB-RS-2 cells.
摘要
已经开发出一种使用猪细胞系IB-RS-2和牛肠道病毒CBV-D作为攻击病毒来检测牛干扰素的方法。该方法基于通过中性红摄取来测量细胞病变效应的评估。该检测方法简单、灵敏且可重复。在IB-RS-2细胞和犊牛肾继代细胞中对不同病毒进行的比较试验表明，在这两种细胞系统中，一种病毒对干扰素的敏感性差异可达1000倍。发现水疱性口炎病毒在IB-RS-2细胞中对干扰素相当不敏感。
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