Toffolo Gianna, Dalla Man Chiara, Cobelli Claudio, Sunehag Agneta L
Department ofl Information Engineering, University of Padova, Padova, Italy.
J Pediatr Endocrinol Metab. 2008 Jan;21(1):31-45. doi: 10.1515/jpem.2008.21.1.31.
Virtually no information is available on glucose fluxes during a meal or glucose ingestion in adolescents.
To use a triple tracer approach to measure rates of appearance of ingested glucose (Ra(ogtt)), endogenous glucose production (EGP) and glucose disappearance (Rd) following an oral glucose bolus in adolescents.
Eleven adolescents (4 M/7 F, 15 +/- 1 yr; 67.3 +/- 4.7 kg; 24 +/-2 kg/m2) underwent a frequent sampled oral glucose tolerance test (OGTT) (labelled with [6,6-2H2]glucose) combined with intravenous infusion of [1-(13)C]glucose and [U-(13)C6]glucose following an overnight fast. Formulas were developed to estimate glucose fluxes using one- or two-compartment models.
During the 7 h following the OGTT bolus, 9.8 +/- 2.3% of the ingested glucose was extracted by the liver, EGP was suppressed by 45 +/- 4% and Rd increased by 21 +/- 5%.
The triple tracer method provided accurate assessment of Ra(ogtt), EGP and Rd fluxes during an OGTT in adolescents. Thus, this method might provide novel insight on postprandial glucose fluxes in children/adolescents under various conditions.
关于青少年在进餐或摄入葡萄糖期间的葡萄糖通量，几乎没有可用信息。
采用三重示踪剂方法测量青少年口服葡萄糖推注后摄入葡萄糖的出现率（Ra(ogtt)）、内源性葡萄糖生成（EGP）和葡萄糖消失率（Rd）。
11名青少年（4名男性/7名女性，15±1岁；67.3±4.7千克；24±2千克/平方米）在禁食过夜后，接受了频繁采样的口服葡萄糖耐量试验（OGTT）（用[6,6-2H2]葡萄糖标记），并静脉输注[1-(13)C]葡萄糖和[U-(13)C6]葡萄糖。开发了使用单室或双室模型估算葡萄糖通量的公式。
在OGTT推注后的7小时内，肝脏提取了9.8±2.3%的摄入葡萄糖，EGP被抑制了45±4%，Rd增加了21±5%。
三重示踪剂方法准确评估了青少年OGTT期间的Ra(ogtt)、EGP和Rd通量。因此，该方法可能为不同条件下儿童/青少年的餐后葡萄糖通量提供新的见解。
