Kocevski Dragana, Tvrdeić Ante
Department of Pharmacology, School of Medicine, University Josip Juraj Strossmayer, Osijek, Croatia.
Coll Antropol. 2008 Jan;32 Suppl 1:93-7.
The hypothesis that repeated measurements during 4 subsequent days affect withdrawal latencies in Hargreaves test was investigated. Paw withdrawal latencies to radiant heat were determined in the control, tramadol or saline group of male Wistar rats. The control group (N=10) had no treatment. Tramadol group (N=7) and saline group (N=7) received one daily intraperitoneal injection of tramadol (15 mg/kg) or saline (0.9% NaCl), respectively. A significant decline in withdrawal latencies was observed in the control group on the day 2 to day 4, when compared to day 1 (p < 0.05 Bonferroni test). In the saline and tramadol groups, latencies remained stable from day 1 to day 4. During the entire testing period withdrawal latencies were 27-50% longer in tramadol group (p < 0.05 ANOVA) compared with the saline group. When compared to the control group, the effect of tramadol, was noted from the second to forth day (p < 0.01 Bonferroni test), but not on the first day. Finally, a tendency to decrement in withdrawal latencies existed on day 1 in the saline group compared with control group, but this difference does not reach significance. We conclude that one day of training affect withdrawal latencies in the Hargreaves test.
研究了在随后4天内进行重复测量是否会影响哈格里夫斯试验中的撤足潜伏期这一假设。在雄性Wistar大鼠的对照组、曲马多组或生理盐水组中测定了对辐射热的撤足潜伏期。对照组（N = 10）未接受任何处理。曲马多组（N = 7）和生理盐水组（N = 7）分别每天腹腔注射一次曲马多（15 mg/kg）或生理盐水（0.9% NaCl）。与第1天相比，对照组在第2天至第4天观察到撤足潜伏期显著下降（Bonferroni检验，p < 0.05）。在生理盐水组和曲马多组中，潜伏期从第1天到第4天保持稳定。在整个测试期间，曲马多组的撤足潜伏期比生理盐水组长27 - 50%（方差分析，p < 0.05）。与对照组相比，曲马多的作用在第2天至第4天观察到（Bonferroni检验，p < 0.01），但在第1天未观察到。最后，与对照组相比，生理盐水组在第1天存在撤足潜伏期下降的趋势，但这种差异未达到显著水平。我们得出结论，一天的训练会影响哈格里夫斯试验中的撤足潜伏期。
