果蝇背腹模式形成基因类 tolloid 与人类骨形态发生蛋白 1 相关。

The Drosophila dorsal-ventral patterning gene tolloid is related to human bone morphogenetic protein 1.

作者信息

Shimell M J, Ferguson E L, Childs S R, O'Connor M B

机构信息

Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.

出版信息

Cell. 1991 Nov 1;67(3):469-81. doi: 10.1016/0092-8674(91)90522-z.

DOI:10.1016/0092-8674(91)90522-z

PMID:1840509

Abstract

Mutations in the Drosophila tolloid (tld) gene lead to a partial transformation of dorsal ectoderm into ventral ectoderm. The null phenotype of tld is similar to, but less severe than decapentaplegic (dpp), a TGF-beta family member required for the formation of all dorsal structures. We have cloned the tld locus by P element tagging. At the blastoderm stage, tld RNA is expressed dorsally, similar to that described for dpp. Analysis of a tld cDNA reveals three sequence motifs: an N terminal region of similarity to a metalloprotease, two EGF-like repeats, and five copies of a repeat found in human complement proteins C1r and C1s. tld sequence is 41% identical to human bone morphogenetic protein 1 (BMP-1); the closest members to dpp within the TGF-beta superfamily are BMP-2 and BMP-4, two other bone morphogenetic proteins. These findings suggest that these genes are members of a signal generating pathway that has been conserved between insects and mammals.

摘要

果蝇类 tolloid（tld）基因的突变会导致背侧外胚层部分转化为腹侧外胚层。tld 的无效表型与 decapentaplegic（dpp）相似，但程度较轻，dpp 是一种 TGF-β 家族成员，是形成所有背侧结构所必需的。我们通过 P 因子标签克隆了 tld 基因座。在囊胚期，tld RNA 在背侧表达，与 dpp 的表达情况相似。对 tld cDNA 的分析揭示了三个序列基序：一个与金属蛋白酶相似的 N 端区域、两个 EGF 样重复序列以及在人类补体蛋白 C1r 和 C1s 中发现的一个重复序列的五个拷贝。tld 序列与人类骨形态发生蛋白 1（BMP-1）有 41%的同一性；在 TGF-β 超家族中，与 dpp 关系最密切的成员是另外两种骨形态发生蛋白 BMP-2 和 BMP-4。这些发现表明，这些基因是昆虫和哺乳动物之间保守的信号产生途径的成员。