Hostinová E, Balanová J, Gasperík J
Slovak Academy of Sciences, Institute of Molecular Biology, Bratislava.
FEMS Microbiol Lett. 1991 Sep 15;67(1):103-8. doi: 10.1016/0378-1097(91)90452-g.
The nucleotide sequence of the 2544-bp PstI fragment carrying the glucoamylase gene of Saccharomycopsis fibuligera KZ, designated as GLA1, has been determined. When compared with the nucleotide sequence of the GLU1 gene one nucleotide substitution was found in the 321- bp of the 5'-flanking region: 24 nucleotides were altered within the 1557 bp of the structural gene causing the deduced protein products of both genes to differ in three amino acids in the signal-peptide region and in eight amino acids of the mature protein. Six nucleotide insertions and 27 substitutions were in the 663 bp of the 3'-flanking region. The gene product expressed and secreted in Saccharomyces cerevisiae into the functional enzyme was not homogeneous. In situ detection of the enzyme in a polyacrylamide gel revealed two dominant and three minor bands.
已确定携带扣囊复膜孢酵母KZ葡糖淀粉酶基因的2544 bp PstI片段（命名为GLA1）的核苷酸序列。与GLU1基因的核苷酸序列相比，在5'侧翼区的321 bp处发现一个核苷酸替换；在结构基因的1557 bp内有24个核苷酸发生改变，导致两个基因推导的蛋白质产物在信号肽区域有三个氨基酸不同，在成熟蛋白中有八个氨基酸不同。在3'侧翼区的663 bp中有六个核苷酸插入和27个替换。该基因产物在酿酒酵母中表达并分泌为功能酶，但并不均一。在聚丙烯酰胺凝胶中对该酶进行原位检测，显示出两条主带和三条次带。
