Bartolomé B, Jubete Y, Martínez E, de la Cruz F
Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
Gene. 1991 Jun 15;102(1):75-8. doi: 10.1016/0378-1119(91)90541-i.
A family of cloning vectors derived from plasmid pACYC184 and, therefore, compatible with pBR322 and its derivatives (especially the pUC family of vectors), is described. They all contain a multiple cloning site (MCS) and the lacZ alpha reporter gene for easy cloning. They have been grouped in three sets: (i) six of the vectors contain a chloramphenicol-resistance (CmR)-encoding gene and each a different MCS with 16 unique restriction sites overall; (ii) another six vectors contain a kanamycin-resistance (KmR)-encoding gene and the same six MCS; and (iii) two CmR vectors that contain the SP6 and T7 promoters flanking the MCS and lacZ alpha reporter gene of pUC18/19.
本文描述了一类源自质粒pACYC184的克隆载体家族，因此与pBR322及其衍生物（特别是pUC载体家族）兼容。它们都含有一个多克隆位点（MCS）和用于便于克隆的lacZα报告基因。它们被分为三组：（i）六个载体含有氯霉素抗性（CmR）编码基因，每个载体有不同的MCS，总共16个独特的限制性酶切位点；（ii）另外六个载体含有卡那霉素抗性（KmR）编码基因和相同的六个MCS；以及（iii）两个CmR载体，其在pUC18/19的MCS和lacZα报告基因两侧含有SP6和T7启动子。
