源自pAL5000和pACYC184的分枝杆菌/大肠杆菌穿梭载体家族的构建：其用于克隆偶然分枝杆菌的抗生素抗性基因。
Construction of a family of Mycobacterium/Escherichia coli shuttle vectors derived from pAL5000 and pACYC184: their use for cloning an antibiotic-resistance gene from Mycobacterium fortuitum.
作者信息
Aínsa J A, Martín C, Cabeza M, De la Cruz F, Mendiola M V
机构信息
Departamento de Microbiología, Universidad de Zaragoza, Spain.
出版信息
Gene. 1996 Oct 17;176(1-2):23-6. doi: 10.1016/0378-1119(96)00202-8.
The pSUM vectors, a family of Mycobacterium/Escherichia coli shuttle cloning vectors derived from the pSU series of vectors are described. They all contain the pACYC184 origin of replication enabling them to replicate in E. coli, the mycobacterial pAL5000 origin of replication and a kanamycin-resistance (KmR) gene which allows selection in both E. coli and mycobacteria. They carry a multiple cloning site (MCS) to facilitate cloning and the lacZ alpha reporter gene for screening inserts in E. coli. A library of total DNA from a strain of Mycobacterium fortuitum was constructed with one of these vectors, pSUM36, and a mycobacterial gentamicin-resistance (GmR) gene was cloned by selecting for GmR in Mycobacterium smegmatis.
描述了pSUM载体，这是一族源自pSU载体系列的分枝杆菌/大肠杆菌穿梭克隆载体。它们都含有pACYC184复制起点，使其能够在大肠杆菌中复制，还含有分枝杆菌pAL5000复制起点以及一个卡那霉素抗性（KmR）基因，该基因允许在大肠杆菌和分枝杆菌中进行筛选。它们带有一个多克隆位点（MCS）以促进克隆，以及用于在大肠杆菌中筛选插入片段的lacZα报告基因。用其中一种载体pSUM36构建了偶然分枝杆菌菌株的总DNA文库，并通过在耻垢分枝杆菌中选择庆大霉素抗性（GmR）来克隆分枝杆菌庆大霉素抗性（GmR）基因。
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