Ye Mei-na, Chen Hong-feng
Department of Breast Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Zhong Xi Yi Jie He Xue Bao. 2008 Apr;6(4):399-404. doi: 10.3736/jcim20080414.
To investigate the effects of Astragalus injection (AI) on basal-like breast cancer cell line MDA-MB-468 and murine bone marrow stromal stem cells (mMSCs).
MDA-MB-468 cells and primary cultured mMSCs were treated by different concentrations of AI, and with untreated MDA-MB-468 cells as blank control. The morphology of cells was observed by phase-contrast inverted microscope and transmission electron microscopy. Cytotoxic effects of AI on MDA-MB-468 cells and mMSCs were evaluated by cell counting kit-8 (CCK-8) assay. Cell cycle and apoptosis of MDA-MB-468 cells induced by AI were measured by flow cytometry. Lactate dehydrogenase (LDH) activity in supernatants was measured by enzymatic colorimetric method. The expressions of epidermal growth factor receptor (EGFR) and p53 protein in MDA-MB-468 cells were evaluated by streptavidin-biotin-peroxidase complex method.
A time-dependent cytotoxic effect of 1 g/ml AI was observed in MDA-MB-468 cells. 1 g/ml AI also had cytotoxic effect on mMSCs, but its effect was not better than cisplatin. 0.1 g/ml AI could promote the proliferation of mMSCs. Different concentrations of AI could all induce the apoptosis of MDA-MB-468 cells. There was no significant difference in LDH activity in the supernatants between blank control group and AI-treated and cisplatin-treated groups. AI could down-regulate the expressions of EGFR and p53 protein.
The effects of AI on MDA-MB-468 cells and mMSCs are related to the concentration of AI, and its mechanism of inhibiting the proliferation of MDA-MB-468 cells may be due to down-regulation of the expressions of EGFR and p53 protein.
探讨黄芪注射液（AI）对基底样乳腺癌细胞系MDA-MB-468和小鼠骨髓基质干细胞（mMSCs）的影响。
用不同浓度的AI处理MDA-MB-468细胞和原代培养的mMSCs，以未处理的MDA-MB-468细胞作为空白对照。通过相差倒置显微镜和透射电子显微镜观察细胞形态。采用细胞计数试剂盒-8（CCK-8）法评估AI对MDA-MB-468细胞和mMSCs的细胞毒性作用。通过流式细胞术检测AI诱导的MDA-MB-468细胞的细胞周期和凋亡情况。采用酶比色法测定上清液中乳酸脱氢酶（LDH）活性。通过链霉亲和素-生物素-过氧化物酶复合物法评估MDA-MB-468细胞中表皮生长因子受体（EGFR）和p53蛋白的表达。
在MDA-MB-468细胞中观察到1 g/ml AI具有时间依赖性细胞毒性作用。1 g/ml AI对mMSCs也有细胞毒性作用，但其作用不比顺铂好。0.1 g/ml AI可促进mMSCs的增殖。不同浓度的AI均可诱导MDA-MB-468细胞凋亡。空白对照组与AI处理组和顺铂处理组之间上清液中LDH活性无显著差异。AI可下调EGFR和p53蛋白的表达。
AI对MDA-MB-468细胞和mMSCs的作用与AI浓度有关，其抑制MDA-MB-468细胞增殖的机制可能是由于下调了EGFR和p53蛋白的表达。
