Deng Ying, Chen Hong-feng
Department of Breast Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Zhong Xi Yi Jie He Xue Bao. 2009 Dec;7(12):1174-80. doi: 10.3736/jcim20091216.
To observe the effects of Astragalus injection, astragaloside IV and formononetin on proliferation and Akt phosphorylation of basal-like human breast carcinoma cell lines MDA-MB-468 and MDA-MB-231.
The effects of different concentrations of Astragalus injection, astragaloside IV and formononetin on proliferation of breast cancer cell lines were assayed by methyl thiazolyl tetrazolium (MTT) assay, and their effects on phospho-Akt were assayed by in-cell Western blot method.
The results of the MTT assay showed that the best concentrations of Astragalus injection, astragaloside IV, formononetin and astragaloside IV plus formononetin were 1 g/mL, 80 microg/mL, 40 microg/mL and 10 microg/mL plus 40 microg/mL respectively. After 1- or 2-day culture, Astragalus injection, astragaloside IV, formononetin and astragaloside IV plus formononetin decreased the expressions of p-Akt (Thr 308) and p-Akt (Ser 473) in MDA-MB-468 cells. Formononetin and astragaloside IV plus formononetin down-regulated the expression of p-Akt (Thr 308) protein in MDA-MB-231 cells after 1- and 2-day culture, but had no effects on the expression of p-Akt (Ser 473) protein in MDA-MB-231 cells.
Astragalus injection, astragaloside IV and formononetin can inhibit proliferation of breast cancer cell lines MDA-MB-468 and MDA-MB-231, and the antiproliferation effects vary according to their concentrations. And the antiproliferation mechanisms may be related to their down-regulation effects on Akt phosphorylation.
观察黄芪注射液、黄芪甲苷IV和芒柄花黄素对基底样人乳腺癌细胞系MDA-MB-468和MDA-MB-231增殖及Akt磷酸化的影响。
采用甲基噻唑基四氮唑（MTT）法检测不同浓度的黄芪注射液、黄芪甲苷IV和芒柄花黄素对乳腺癌细胞系增殖的影响，采用细胞内Western印迹法检测其对磷酸化Akt的影响。
MTT法结果显示，黄芪注射液、黄芪甲苷IV、芒柄花黄素及黄芪甲苷IV加芒柄花黄素的最佳浓度分别为1 g/mL、80 μg/mL、40 μg/mL和10 μg/mL加40 μg/mL。培养1天或2天后，黄芪注射液、黄芪甲苷IV、芒柄花黄素及黄芪甲苷IV加芒柄花黄素降低了MDA-MB-468细胞中p-Akt（苏氨酸308）和p-Akt（丝氨酸473）的表达。培养1天和2天后，芒柄花黄素及黄芪甲苷IV加芒柄花黄素下调了MDA-MB-231细胞中p-Akt（苏氨酸308）蛋白的表达，但对MDA-MB-231细胞中p-Akt（丝氨酸473）蛋白的表达无影响。
黄芪注射液、黄芪甲苷IV和芒柄花黄素可抑制乳腺癌细胞系MDA-MB-468和MDA-MB-231的增殖，并根据其浓度不同产生不同的抗增殖作用。其抗增殖机制可能与其下调Akt磷酸化有关。
