Parmentier M, Passage E, Mattei M G, Vassart G
IRIBHN, Université libre de Bruxelles, Belgium.
Genomics. 1991 Nov;11(3):760-2. doi: 10.1016/0888-7543(91)90086-t.
A human genomic library was screened with a 30-base oligomer corresponding to the 5' end of the human calretinin cDNA. A clone that contains a minisatellite composed of 21 imperfect repeats of a 37-bp sequence was isolated. The consensus (GAGGGAGGAACTGGGACGCGTGCATGTTTGCATTCTC) incidentally shares 14 consecutive matches with the oligomer used as a probe, and it was shown that the clone did not belong to the calretinin locus. The minisatellite, named K29, was used as a probe on Southern blots at high stringency. After HaeIII, MboI, or HinfI digestion, it detected a single hypervariable locus, with 65% heterozygosity among Caucasian individuals. The probe used at low stringency revealed a fingerprint, with an average of four bands in addition to the locus-specific pattern. Mendelian inheritance was assessed on pedigrees. The K29 minisatellite was mapped by in situ hybridization to the very end of the long arm of chromosome 2 (2q37.3 band), at close proximity of the Fra2J locus, and is referred to as the D2S88 locus in the genome database.
用人钙视网膜蛋白cDNA 5'端对应的30个碱基的寡聚物筛选人基因组文库。分离出一个包含由37个碱基序列的21个不完全重复组成的小卫星的克隆。该共有序列（GAGGGAGGAACTGGGACGCGTGCATGTTTGCATTCTC）偶然与用作探针的寡聚物有14个连续匹配，并且显示该克隆不属于钙视网膜蛋白基因座。这个名为K29的小卫星在高严谨度的Southern印迹中用作探针。用HaeIII、MboI或HinfI消化后，它检测到一个单一的高变位点，在高加索个体中有65%的杂合性。在低严谨度下使用的探针显示出一种指纹图谱，除了位点特异性模式外平均还有四条带。在系谱上评估孟德尔遗传。通过原位杂交将K29小卫星定位到2号染色体长臂的末端（2q37.3带），靠近Fra2J基因座，在基因组数据库中被称为D2S88基因座。
