Baldini A, Archidiacono N, Carbone R, Bolino A, Shridhar V, Miller O J, Miller D A, Ward D C, Rocchi M
Department of Human Genetics, Yale University School of Medicine, New Haven, CT.
Cytogenet Cell Genet. 1992;59(1):12-6. doi: 10.1159/000133188.
We have isolated and characterized a human genomic DNA clone (PZ20, locus D20Z2) that identifies, under high-stringency hybridization conditions, an alphoid DNA subset specific for chromosome 20. The specificity was determined using fluorescence in situ hybridization. Sequence analysis confirmed our previously reported data on the great similarity between the chromosome 20 and chromosome 2 alphoid subsets. Comparative mapping of pZ20 on chimpanzee and gorilla chromosomes, also performed under high-stringency conditions, indicates that the alphoid subset has ancestral sequences on chimpanzee chromosome 11 and gorilla chromosome 19. However, no hybridization was observed to chromosomes 21 in the great apes, the homolog of human chromosome 20.
我们分离并鉴定了一个人类基因组DNA克隆（PZ20，基因座D20Z2），在高严格度杂交条件下，该克隆可识别出20号染色体特有的一类α卫星DNA亚群。通过荧光原位杂交确定了其特异性。序列分析证实了我们之前报道的关于20号染色体和2号染色体α卫星亚群之间高度相似性的数据。在高严格度条件下对黑猩猩和大猩猩染色体上的pZ20进行的比较定位表明，该α卫星亚群在黑猩猩11号染色体和大猩猩19号染色体上有祖先序列。然而，在大猩猩中未观察到与21号染色体（人类20号染色体的同源染色体）的杂交。
