Tang Kaijie, Chen Shangwei, Gu Xiaohong, Wang Haijun, Dai Jun, Tang Jian
State Key Laboratory of Food Science & Technology, Jiangnan University, Jiangsu, Wuxi 214122, China.
Anal Chim Acta. 2008 Apr 28;614(1):112-8. doi: 10.1016/j.aca.2008.03.018. Epub 2008 Mar 14.
A pre-treatment methodology based on the molecularly imprinted solid phase extraction (MI-SPE) procedure was developed for the determination of bensulfuron-methyl (BSM), tribenuron-methyl (TBM), metsulfuron-methyl (MSM) and nicosulfuron (NS) in soybean samples. A molecular imprinted polymer (MIP) was prepared by precipitation polymerization using BSM as the template molecule, alpha-methacrylic acid (MAA) as the functional monomer, trimethylolpropane trimethacrylate (TRIM) as the cross-linker and dichloromethane as the porogen. The binding behaviors of the template BSM and its analogues on the MIP were evaluated by high performance liquid chromatography (HPLC). Then, solid phase extraction (SPE) with a BSM molecularly imprinted polymer (BSM-MIP) as adsorbent was investigated and the optimum loading, washing, and eluting conditions for MI-SPE of the selected BSM, MSM, TBM, and NS were established. The optimized MI-SPE procedure was used to extract the sulfonylureas and a high recovery was obtained in the soybean samples.
建立了一种基于分子印迹固相萃取（MI-SPE）的预处理方法，用于测定大豆样品中的苄嘧磺隆（BSM）、苯磺隆（TBM）、甲磺隆（MSM）和烟嘧磺隆（NS）。以BSM为模板分子，α-甲基丙烯酸（MAA）为功能单体，三羟甲基丙烷三甲基丙烯酸酯（TRIM）为交联剂，二氯甲烷为致孔剂，通过沉淀聚合法制备了分子印迹聚合物（MIP）。采用高效液相色谱（HPLC）评价了模板分子BSM及其类似物在MIP上的结合行为。然后，研究了以BSM分子印迹聚合物（BSM-MIP）为吸附剂的固相萃取（SPE），并确定了所选BSM、MSM、TBM和NS的MI-SPE最佳上样、洗涤和洗脱条件。采用优化后的MI-SPE方法萃取磺酰脲类化合物，在大豆样品中获得了较高的回收率。
