Mori Kyoko, Abe Ken-Ichi, Dansako Hiromichi, Ariumi Yasuo, Ikeda Masanori, Kato Nobuyuki
Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
Biochem Biophys Res Commun. 2008 Jun 20;371(1):104-9. doi: 10.1016/j.bbrc.2008.04.005. Epub 2008 Apr 10.
We report for the first time a new RNA replication system with a hepatitis C virus (HCV) strain (AH1) derived from a patient with acute hepatitis C. Using an HCV replicon RNA library constructed with the AH1 strain (genotype 1b), we first established a cloned cell line, sAH1, harboring the HCV replicon. Cured cells obtained with interferon treatment of sAH1 cells were used for transfection with genome-length HCV RNA possessing four mutations found in sAH1 replicon. Consequently, one cloned cell line, AH1, supporting efficient replication of genome-length HCV RNA was obtained. By the comparison of AH1 cells with the O cells supporting genome-length HCV RNA (HCV-O strain) replication, we found different anti-HCV profiles of interferon-gamma and cyclosporine A between AH1 and O cells. Reporter assay analysis suggests that the diverse effects of interferon-gamma are due to the difference in HCV strains, but not the cellular environment.
我们首次报道了一种新的RNA复制系统，该系统使用的是来自一名急性丙型肝炎患者的丙型肝炎病毒（HCV）毒株（AH1）。利用用AH1毒株（1b基因型）构建的HCV复制子RNA文库，我们首先建立了一个携带HCV复制子的克隆细胞系sAH1。用干扰素处理sAH1细胞获得的治愈细胞用于转染具有在sAH1复制子中发现的四个突变的全长HCV RNA。结果，获得了一个支持全长HCV RNA高效复制的克隆细胞系AH1。通过将AH1细胞与支持全长HCV RNA（HCV-O毒株）复制的O细胞进行比较，我们发现AH1细胞和O细胞之间干扰素-γ和环孢素A的抗HCV谱不同。报告基因分析表明，干扰素-γ的不同作用是由于HCV毒株的差异，而非细胞环境的差异。
