Jia Yin-tang, Wei Lai, Jiang Dong, Cong Xu, Fei Ran
Department of Infectious Diseases, First Hospital, Medical Sciences of Shanxi University, Taiyuan 030001, China.
Zhonghua Gan Zang Bing Za Zhi. 2006 Jan;14(1):7-10.
To study the effects of interferon-gamma and interferon-gamma combined with interferon-alpha on HCV RNA replication and the possible mediators of interferon-gamma anti-HCV in vitro.
An HCV replicon cell culture system was established and the cells were treated with interferon-gamma or interferon-gamma combined with interferon-alpha. HCV RNA levels in the cells were evaluated by semi-quantitative RT-PCR and real-time PCR, and the levels of NS5A protein were examined by Western blot.
Interferon-gamma could inhibit HCV RNA replication and NS5A protein expression effectively; The anti-HCV effects of interferon-gamma were both in time-dependent and does-dependent manners; Pretreating the cells with interferon-gamma could significantly enhance the antiviral effects of interferon-alpha; The expressions of IRF-1, 2'5'-OAS1(p46), 2'5'-OAS2(p69), ISGF3gamma and STAT1 were significantly increased after interferon-gamma treatment.
Interferon-gamma has a direct inhibitory effect on HCV replicon RNA replication and NS5A expression, and both are in a dose and time dependent manner; Interferon-gamma has a synergistic anti-HCV effect with interferon-alpha. IRF-1, 2'5'-OAS1(p46), 2'5'-OAS2(p69) and ISGF3gamma may mediate the anti-HCV effects of interferon-gamma.
研究γ干扰素及γ干扰素联合α干扰素对丙型肝炎病毒（HCV）RNA复制的影响以及γ干扰素体外抗HCV的可能介导因子。
建立HCV复制子细胞培养系统，用γ干扰素或γ干扰素联合α干扰素处理细胞。通过半定量逆转录聚合酶链反应（RT-PCR）和实时荧光定量PCR评估细胞内HCV RNA水平，并用蛋白质印迹法检测NS5A蛋白水平。
γ干扰素能有效抑制HCV RNA复制和NS5A蛋白表达；γ干扰素的抗HCV作用呈时间和剂量依赖性；用γ干扰素预处理细胞可显著增强α干扰素的抗病毒作用；γ干扰素处理后，干扰素调节因子1（IRF-1）、2',5'-寡腺苷酸合成酶1（2'5'-OAS1，p46）、2'5'-寡腺苷酸合成酶2（2'5'-OAS2，p69）、干扰素刺激基因因子3γ（ISGF3γ）和信号转导子和转录激活子1（STAT1）的表达显著增加。
γ干扰素对HCV复制子RNA复制和NS5A表达有直接抑制作用，且均呈剂量和时间依赖性；γ干扰素与α干扰素具有协同抗HCV作用。IRF-1、2'5'-OAS1（p46）、2'5'-OAS2（p69）和ISGF3γ可能介导γ干扰素的抗HCV作用。
