Wang Ke, Huang Lihua, Kong Jian, Zhang Xiaowei
State Key Laboratory for Microbial Technology, Shandong University, No. 27 Shanda Nan Road, Jinan, Shandong, China.
J Virol Methods. 2008 Jun;150(1-2):1-6. doi: 10.1016/j.jviromet.2008.02.014. Epub 2008 Apr 11.
Diseases associated with porcine circovirus type 2 (PCV2) infections are becoming a major problem for the swine industry worldwide. The capsid protein (Cap) of PCV2 is an antigen important for both early diagnosis and development of vaccines. In this study, Lactococcus lactis was used as vehicle to deliver the PCV2 antigen in an attempt to develop oral vaccine. A cap gene with a deleted nuclear localization signal sequence (dcap) was cloned into an Escherichia coli/L. lactis shuttle vector pSEC: LEISS under the control of a nisin promoter. Intracellular and extracellular expression of the dCap was confirmed by Western blot analysis. Significantly higher levels of PCV2-specific IgG in the sera of mice were observed upon oral administration of strain cultures expressing the PCV2 antigen. These results suggest that it is feasible to use L. lactis as an antigen delivery vehicle for developing oral vaccines against PCV2 infection.
与猪圆环病毒2型（PCV2）感染相关的疾病正成为全球养猪业的一个主要问题。PCV2的衣壳蛋白（Cap）是早期诊断和疫苗开发的重要抗原。在本研究中，乳酸乳球菌被用作载体来递送PCV2抗原，试图开发口服疫苗。将一个缺失核定位信号序列的cap基因（dcap）克隆到一个在乳酸链球菌素启动子控制下的大肠杆菌/乳酸乳球菌穿梭载体pSEC:LEISS中。通过蛋白质免疫印迹分析证实了dCap在细胞内和细胞外的表达。口服表达PCV2抗原的菌株培养物后，在小鼠血清中观察到PCV2特异性IgG水平显著升高。这些结果表明，使用乳酸乳球菌作为抗原递送载体来开发针对PCV2感染的口服疫苗是可行的。
