Osman Osmali, Fong Mun Yik, Devi Shamala
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia.
Virus Res. 2008 Jul;135(1):48-52. doi: 10.1016/j.virusres.2008.02.006. Epub 2008 Apr 11.
In a previous study, we have reported the detection and isolation of dengue virus in Brunei (Osman, O., Fong, M.Y., Devi, S., 2007. A preliminary study of dengue infection in Brunei. JJID 60 (4), 205-208). DEN-2 was the predominant serotype followed by DEN-1. The full genomic sequences of 3 DEN-2 viruses isolated during the 2005-2006 dengue incident in Brunei were determined. Twenty-five primer sets were designed to amplify contiguous overlapping fragments of approximately 500-600 base pairs spanning the entire sequence of the viral genome. The amplified PCR products were sent for sequencing and their nucleotides and the deduced amino acids were determined. All three DEN-2 virus isolated were clustered in the Cosmopolitan genotype of the DEN-2 classification by Twiddy et al. This work constitutes the first complete genetic characterization of three Brunei DEN-2 virus strains.
在之前的一项研究中，我们报告了在文莱检测和分离登革病毒的情况（奥斯曼，O.，方，M.Y.，德维，S.，2007年。文莱登革热感染的初步研究。《日本传染病杂志》60(4)，205 - 208）。DEN - 2是主要血清型，其次是DEN - 1。测定了在2005 - 2006年文莱登革热疫情期间分离出的3株DEN - 2病毒的全基因组序列。设计了25对引物，用于扩增跨越病毒基因组全序列的约500 - 600个碱基对的连续重叠片段。将扩增的PCR产物送去测序，并确定其核苷酸和推导的氨基酸。根据Twiddy等人的分类，分离出的所有3株DEN - 2病毒都聚集在DEN - 2分类的泛在基因型中。这项工作构成了对3株文莱DEN - 2病毒株的首次完整基因特征分析。
