Laille Manola, Roche Claudine
Laboratoire de Recherche en Virologie Médicale, Institut Louis Malardé, PO Box 30, 98713 Papeete Tahiti, Polynésie Française.
Am J Trop Med Hyg. 2004 Oct;71(4):478-84.
Dengue (DEN) is the leading arboviral infection of humans, with 100 million cases annually in the tropical areas of the world. The recent severe DEN-1 epidemic in French Polynesia in 2001, with an incidence rate of 16% and more than 45% of the cases with dengue hemorrhagic fever/dengue shock syndrome among 1,400 hospitalized children and eight fatalities, led us to study this new circulating strain. The entire envelope (E) gene of two French Polynesian DEN-1 virus isolates from the two epidemics of 1988-1989 (FP89) and 2001 (FP01) were sequenced and compared with 29 published DEN-1 virus E gene sequences. Phylogenetic relationships showed that the FP89 strain belonged to genotype V and the FP01 strain to genotype IV based on studies on the same region of DEN-1 virus genome (1,485 nucleotides). The recent dengue epidemic in French Polynesia in 2001 was probably due to the introduction of a new DEN-1 virus from Southeast Asia, since the minimum nucleotide divergence was 3.3% with A88, the Indonesian strain isolated in 1988 in Jakarta.
登革热（DEN）是人类最主要的虫媒病毒感染疾病，在世界热带地区每年有1亿例病例。2001年法属波利尼西亚最近发生的严重登革热1型疫情，在1400名住院儿童中发病率为16%，登革出血热/登革休克综合征病例超过45%，并有8例死亡，这促使我们对这种新的流行毒株进行研究。对来自1988 - 1989年（FP89）和2001年（FP01）两次疫情的两株法属波利尼西亚登革热1型病毒分离株的整个包膜（E）基因进行了测序，并与29个已发表的登革热1型病毒E基因序列进行了比较。基于对登革热1型病毒基因组同一区域（1485个核苷酸）的研究，系统发育关系表明，FP89毒株属于基因型V，FP01毒株属于基因型IV。2001年法属波利尼西亚最近的登革热疫情可能是由于从东南亚引入了一种新的登革热1型病毒，因为与1988年在雅加达分离的印度尼西亚毒株A88的最小核苷酸差异为3.3%。
