Islam Mohammed Alimul, Ahmed Muzahed Uddin, Begum Nasima, Chowdhury Naseem Akhtar, Khan Afjal Hossain, Parquet Maria del Carmen, Bipolo Sophie, Inoue Shingo, Hasebe Futoshi, Suzuki Yasuo, Morita Kouichi
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.
Jpn J Infect Dis. 2006 Apr;59(2):85-91.
During the febrile illness epidemic in Bangladesh in 2002, 58 people died out of the 6,132 affected. Two hundred hospitalized patients were analyzed clinically, serologically and virologically to determine the features of this dengue infection. Among the 10- to 70-year-old age group of the 200 clinically suspected dengue patients, 100 (50%) were confirmed as dengue cases by virus isolation and dengue IgM-capture ELISA. Of the 100 dengue-confirmed cases, the mean age was 29.0 (+/-12.4). The possible dengue secondary infection rate determined by Flavivirus IgG-indirect ELISA was 78% in 2002. Eight dengue virus strains were isolated, representing the first dengue virus isolation in the country, and all of the strains were dengue virus type-3 (DEN-3). Sequence data for the envelope gene of the DEN-3 Bangladeshi isolates were used in a phylogenetic comparison with DEN-3 from other countries. A phylogenetic analysis revealed that all 8 strains of DEN-3 were clustered within a well-supported independent sub-cluster of genotype II and were closely related to the Thai isolates from the 1990s. Therefore, it is likely that the currently circulating DEN-3 viruses entered Bangladesh from neighboring countries.
2002年孟加拉国发热性疾病流行期间，6132名感染者中有58人死亡。对200名住院患者进行了临床、血清学和病毒学分析，以确定此次登革热感染的特征。在200名临床疑似登革热患者的10至70岁年龄组中，100人（50%）通过病毒分离和登革热IgM捕获ELISA确诊为登革热病例。在100例确诊登革热病例中，平均年龄为29.0岁（±12.4）。2002年通过黄病毒IgG间接ELISA确定的可能的登革热二次感染率为78%。分离出8株登革热病毒株，这是该国首次分离出登革热病毒，所有毒株均为3型登革热病毒（DEN-3）。将DEN-3孟加拉国分离株包膜基因的序列数据与其他国家的DEN-3进行系统发育比较。系统发育分析表明，所有8株DEN-3均聚集在基因型II的一个得到充分支持的独立亚群内，并且与20世纪90年代的泰国分离株密切相关。因此，目前在孟加拉国传播的DEN-3病毒很可能是从邻国传入的。
