Chen Wei-liang, Yang Lan, Zeng Shu-guang, Wang Yong-jie
Department of Oral and Maxillofacial Surgery, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, PR China.
Br J Oral Maxillofac Surg. 2008 Sep;46(6):435-8. doi: 10.1016/j.bjoms.2008.02.009. Epub 2008 Apr 11.
This study used RNA interference (RNAi) to explore the effect of NO and inducible nitric oxide synthase (iNOS) on apoptosis and proliferation in the tongue squamous carcinoma cell line Tca8113. Tca8113 cells were transfected with the plasmid pGenesil-1, which expresses iNOS short hairpin RNA (shRNA), or the negative control plasmid pSilencer-HK, and the transfected cells were compared with untransfected cells. The expression of iNOS was detected by histochemistry, and apoptosis was detected by flow cytometry. The expression of iNOS was significantly lower in the pSilencer-iNOS group than in the pSilencer-HK and empty control groups. The apoptosis rate was significantly higher in the pSilencer-iNOS group than in the pSilencer-HK and empty control groups. Growth monitoring showed that proliferation was also inhibited in cells transfected with pSilencer-iNOS. RNAi gene silencing decreased iNOS gene expression, induced apoptosis, and suppressed proliferation in Tca8113 cells.
本研究采用RNA干扰（RNAi）技术，探讨一氧化氮（NO）和诱导型一氧化氮合酶（iNOS）对舌鳞状癌细胞系Tca8113凋亡及增殖的影响。将表达iNOS短发夹RNA（shRNA）的质粒pGenesil-1或阴性对照质粒pSilencer-HK转染至Tca8113细胞，并将转染后的细胞与未转染细胞进行比较。采用组织化学法检测iNOS的表达，采用流式细胞术检测细胞凋亡情况。pSilencer-iNOS组中iNOS的表达明显低于pSilencer-HK组和空载体对照组。pSilencer-iNOS组的凋亡率明显高于pSilencer-HK组和空载体对照组。生长监测显示，转染pSilencer-iNOS的细胞增殖也受到抑制。RNAi基因沉默可降低Tca8113细胞中iNOS基因的表达，诱导细胞凋亡并抑制细胞增殖。
