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用血清学方法比较伊拉克纳杰夫省农村和城市地区的患病率。

Comparisons of Prevalence in Rural and Urban Areas of Al-Najaf Province of Iraq Using Serological Methods.

机构信息

Faculty of Science, University of Kufa, Kufa, Iraq.

出版信息

Arch Razi Inst. 2021 Dec 30;76(6):1695-1701. doi: 10.22092/ari.2021.356315.1822. eCollection 2021 Dec.

Abstract

() is an intracellular protozoan parasite that multiplies within the host cell and causes the disease toxoplasmosis. is distributed worldwide and is capable of infecting almost all warm-blooded animals. The current study was conducted in several urban and rural regions in AL-Najaf province of Iraq from September 2020 to April 2021. In total, 190 blood samples were collected and screened for IgG and IgM antibodies using Rapid Diagnostic immunochromatographic test and the enzyme-linked immunosorbent assay (ELISA). These two tests were performed on 5 ml of blood samples. The results of the ICT test showed that 80 (42.1%) samples were positive for IgG; however, no IgM positive sample was recorded. The results of ELISA revealed that 27 (33.7%) and 4 (5%) samples were positive for IgG and IgM antibodies, respectively. The estimated incidence of toxoplasmosis increased significantly in the 21-30 years age group and females (P<0.05), compared to other groups. The wide prevalence of toxoplasmosis was observed in Iraq, especially in Najaf province, which was reflected in the results of the study after taking random samples from different places with no symptoms of the disease. Therefore, all members of the community should undergo periodic examinations to diagnose possible infection through the most accurate tests.

摘要

刚地弓形虫是一种细胞内的原生动物寄生虫,在宿主细胞内繁殖并引起疾病弓形体病。它分布于世界各地,几乎能够感染所有温血动物。本研究于 2020 年 9 月至 2021 年 4 月在伊拉克纳杰夫省的几个城乡地区进行。总共采集了 190 份血样,并使用快速诊断免疫层析试验和酶联免疫吸附试验(ELISA)对 IgG 和 IgM 抗体进行筛查。这两种检测都在 5ml 的血样上进行。ICT 检测结果显示,80 份(42.1%)样本 IgG 呈阳性;但未记录到 IgM 阳性样本。ELISA 检测结果显示,27 份(33.7%)和 4 份(5%)样本 IgG 和 IgM 抗体分别呈阳性。与其他组相比,21-30 岁年龄组和女性的弓形虫病发病率估计显著增加(P<0.05)。在伊拉克,尤其是在纳杰夫省,广泛存在弓形体病,这在从不同无疾病症状的地方随机取样并进行研究后,反映在结果中。因此,社区的所有成员都应该进行定期检查,通过最准确的检测来诊断可能的感染。

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