巴西坚果（Bertholletia excelsa）2S白蛋白编码基因的分离、特性鉴定及表达
Isolation, characterization and expression of a gene coding for a 2S albumin from Bertholletia excelsa (Brazil nut).
作者信息
Gander E S, Holmstroem K O, De Paiva G R, De Castro L A, Carneiro M, Grossi de Sá M F
机构信息
S.A.I.N., Brasilia-DF, Brazil.
出版信息
Plant Mol Biol. 1991 Mar;16(3):437-48. doi: 10.1007/BF00023994.
Two genes, BE2S1 and BE2S2, coding for methionine-rich albumins of Brazil nut (Bertholletia excelsa H.B.K.) have been cloned and their sequence determined. The genes are members of a multigene family and one of them, i.e. BE2S1, codes for one of the dominant 2S isoforms. Its expression is highly regulated during seed development and with respect to tissue specificity. Sequence analysis has shown that the genes contain one intron and that the promoter of BE2S1 shows a canonical TATA motif. The transcription initiation site is located 26 nucleotides downstream from the TATA box. Sequence comparison of the promoter regions of 2S genes from Brassica napus, Arabidopsis thaliana and B. excelsa revealed the presence of TGCA palindromic sequence that appear to be arranged in a 2S-specific manner.
编码巴西坚果（伯托莱氏坚果，Bertholletia excelsa H.B.K.）富含蛋氨酸白蛋白的两个基因BE2S1和BE2S2已被克隆并测定了其序列。这些基因是一个多基因家族的成员，其中之一即BE2S1编码一种主要的2S同工型。其表达在种子发育过程中以及在组织特异性方面受到高度调控。序列分析表明，这些基因含有一个内含子，并且BE2S1的启动子显示出一个典型的TATA基序。转录起始位点位于TATA框下游26个核苷酸处。对来自甘蓝型油菜、拟南芥和巴西坚果的2S基因启动子区域的序列比较揭示了TGCA回文序列的存在，这些序列似乎以2S特异性方式排列。
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