• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过根癌农杆菌介导转化法获得的苎麻（Boehmeria nivea Gaud.）转基因植株。

Transgenic plants of ramie (Boehmeria nivea Gaud.) obtained by Agrobacterium mediated transformation.

机构信息

EMBRAPA/CENARGEN, SAIN Parque Rural, C.P. 02372, 70849-970, Brasília DF, Brasil.

出版信息

Plant Cell Rep. 1993 Sep;12(11):625-8. doi: 10.1007/BF00232812.

DOI:10.1007/BF00232812
PMID:24201876
Abstract

A regeneration and transformation protocol for ramie (Boehmeria nivea Gaud.) is presented. Regeneration was obtained from leaf discs placed on solid B-5 medium (Gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. Co-cultivation of leaf discs with Agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by Southern blot and analysis of expression of the GUS-marker gene.

摘要

现介绍一种苎麻（Boehmeria nivea Gaud.）的再生和转化方案。通过在含有适当浓度生长素和细胞分裂素的 B-5 固体培养基（Gamborg 等人，1968 年）上放置叶片圆盘获得再生。叶片圆盘与根癌农杆菌共培养，随后进行再生，通过 Southern 印迹和 GUS 标记基因表达分析，得到了转基因植株。

相似文献

1
Transgenic plants of ramie (Boehmeria nivea Gaud.) obtained by Agrobacterium mediated transformation.通过根癌农杆菌介导转化法获得的苎麻（Boehmeria nivea Gaud.）转基因植株。
Plant Cell Rep. 1993 Sep;12(11):625-8. doi: 10.1007/BF00232812.
2
Agrobacterium-mediated genetic transformation and regeneration of transgenic plants using leaf midribs as explants in ramie [Boehmeria nivea (L.) Gaud].利用苎麻[Boehmeria nivea (L.) Gaud]叶片中脉作为外植体，通过农杆菌介导进行遗传转化及转基因植株再生
Mol Biol Rep. 2014 May;41(5):3257-69. doi: 10.1007/s11033-014-3188-4. Epub 2014 Feb 2.
3
Transgenic ramie [Boehmeria nivea (L.) Gaud.]: factors affecting the efficiency of Agrobacterium tumefaciens-mediated transformation and regeneration.转基因苎麻（Boehmeria nivea（L.）Gaud.）：影响农杆菌介导转化和再生效率的因素。
Plant Cell Rep. 2009 Sep;28(9):1319-27. doi: 10.1007/s00299-009-0732-0. Epub 2009 Jun 16.
4
Retraction. Transgenic ramie [Boehmeria nivea (L.) Gaud.]: factors affecting the efficiency of Agrobacterium tumefaciens-mediated transformation and regeneration.撤回。转基因苎麻[苎麻（L.）高氏]：影响根癌农杆菌介导的转化和再生效率的因素。
Plant Cell Rep. 2009 Nov;28(11):1767. doi: 10.1007/s00299-009-0773-4.
5
Senescence is delayed when ramie ( L.) is transformed with the isopentyl transferase () gene under control of the SAG12 promoter.当苎麻（苎麻属）在SAG12启动子控制下用异戊烯基转移酶（）基因转化时，衰老被延迟。
FEBS Open Bio. 2017 Mar 31;7(5):636-644. doi: 10.1002/2211-5463.12191. eCollection 2017 May.
6
Transcript profiling reveals auxin and cytokinin signaling pathways and transcription regulation during in vitro organogenesis of Ramie (Boehmeria nivea L. Gaud).转录谱分析揭示了苎麻（Boehmeria nivea L. Gaud）离体器官发生过程中的生长素和细胞分裂素信号通路及转录调控。
PLoS One. 2014 Nov 21;9(11):e113768. doi: 10.1371/journal.pone.0113768. eCollection 2014.
7
Transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with ramie (Boehmeria nivea (L.) Gaud.) leaf color formation.转录组和代谢组分析揭示了与苎麻（Boehmeria nivea（L.）Gaud.）叶片颜色形成相关的花青素生物合成途径。
BMC Genomics. 2021 Sep 22;22(1):684. doi: 10.1186/s12864-021-08007-0.
8
Assessment of ramie leaf (Boehmeria nivea L. gaud) as an animal feed supplement in P.R. China.中国苎麻叶（苎麻）作为动物饲料添加剂的评估
Trop Anim Health Prod. 2020 Jan;52(1):115-121. doi: 10.1007/s11250-019-01997-w. Epub 2019 Jul 4.
9
Complete chloroplast genome sequence of the medicinal plant ramie ( L. gaud) and its phylogenetic relationship to other Urticaceae species.药用植物苎麻（Boehmeria nivea (L.) Gaud.）的叶绿体全基因组序列及其与荨麻科其他物种的系统发育关系。
Mitochondrial DNA B Resour. 2021 Mar 22;6(3):1136-1137. doi: 10.1080/23802359.2021.1878959.
10
Promoter - From Ramie ( L. Gaud.) Can Drive Phloem-Specific GUS Expression in .启动子 - 来自苎麻（L. Gaud.），可驱动韧皮部特异性GUS在……中的表达。
Front Genet. 2020 Dec 16;11:553265. doi: 10.3389/fgene.2020.553265. eCollection 2020.

引用本文的文献

1
Transcript profiling reveals auxin and cytokinin signaling pathways and transcription regulation during in vitro organogenesis of Ramie (Boehmeria nivea L. Gaud).转录谱分析揭示了苎麻（Boehmeria nivea L. Gaud）离体器官发生过程中的生长素和细胞分裂素信号通路及转录调控。
PLoS One. 2014 Nov 21;9(11):e113768. doi: 10.1371/journal.pone.0113768. eCollection 2014.
2
Agrobacterium-mediated genetic transformation and regeneration of transgenic plants using leaf midribs as explants in ramie [Boehmeria nivea (L.) Gaud].利用苎麻[Boehmeria nivea (L.) Gaud]叶片中脉作为外植体，通过农杆菌介导进行遗传转化及转基因植株再生
Mol Biol Rep. 2014 May;41(5):3257-69. doi: 10.1007/s11033-014-3188-4. Epub 2014 Feb 2.
3

本文引用的文献

1
Protein fusions with the kanamycin resistance gene from transposon Tn5.与转座子Tn5的卡那霉素抗性基因的蛋白质融合体。
EMBO J. 1984 Dec 20;3(13):3317-22. doi: 10.1002/j.1460-2075.1984.tb02297.x.
2
Nutrient requirements of suspension cultures of soybean root cells.大豆根细胞悬浮培养的营养需求。
Exp Cell Res. 1968 Apr;50(1):151-8. doi: 10.1016/0014-4827(68)90403-5.
3
Isolation, characterization and expression of a gene coding for a 2S albumin from Bertholletia excelsa (Brazil nut).巴西坚果（Bertholletia excelsa）2S白蛋白编码基因的分离、特性鉴定及表达
Transgenic ramie [Boehmeria nivea (L.) Gaud.]: factors affecting the efficiency of Agrobacterium tumefaciens-mediated transformation and regeneration.
转基因苎麻（Boehmeria nivea（L.）Gaud.）：影响农杆菌介导转化和再生效率的因素。
Plant Cell Rep. 2009 Sep;28(9):1319-27. doi: 10.1007/s00299-009-0732-0. Epub 2009 Jun 16.
Plant Mol Biol. 1991 Mar;16(3):437-48. doi: 10.1007/BF00023994.