血小板反应蛋白-1的表达与实验性肝硬化中的血管生成相关。
Thrombospondin-1 expression correlates with angiogenesis in experimental cirrhosis.
作者信息
Elpek Gulsum-Ozlem, Gokhan Guzide-Ayse, Bozova Sevgi
机构信息
Akdeniz University, Medical School, Department of Pathology, Antalya 07070, Turkey.
出版信息
World J Gastroenterol. 2008 Apr 14;14(14):2213-7. doi: 10.3748/wjg.14.2213.
AIM
To investigate the significance of Thrombospondin-1 (TSP-1) expression and its relationship with angiogenesis during experimental fibrosis.
METHODS
Cirrhosis was induced in male Wistar rats by intraperitoneal administration of diethyl nitrosamine (DEN). The serial sections from liver tissues were stained with anti-CD34 and anti-TSP-1 antibodies before being quantitated by light microscopy.
RESULTS
Our results showed that of TSP-1 expression gradually increases according to the severity of fibrosis (P < 0.05). Moreover, TSP-1 expression was found to be correlated with angiogenesis (P < 0.05).
CONCLUSION
The correlative evidence of the link between TSP-1 and fibrosis or angiogenesis provided by this study suggests that besides its role as a strong promoter of transforming growth factor-beta 1 (TGF-beta 1), TSP-1 might have an additional role in liver fibrogenesis by stimulating angiogenesis and this protein could be a potential target to prevent fibrogenesis in chronic inflammatory diseases of the liver.
目的
研究血小板反应蛋白-1（TSP-1）表达的意义及其在实验性肝纤维化过程中与血管生成的关系。
方法
通过腹腔注射二乙基亚硝胺（DEN）诱导雄性Wistar大鼠肝硬化。肝组织连续切片用抗CD34和抗TSP-1抗体染色，然后通过光学显微镜进行定量分析。
结果
我们的结果显示，TSP-1表达随着肝纤维化严重程度的增加而逐渐升高（P < 0.05）。此外，发现TSP-1表达与血管生成相关（P < 0.05）。
结论
本研究提供的TSP-1与肝纤维化或血管生成之间联系的相关证据表明，除了作为转化生长因子-β1（TGF-β1）的强促进剂作用外，TSP-1可能通过刺激血管生成在肝纤维化形成中发挥额外作用，并且该蛋白可能是预防慢性肝脏炎症性疾病中纤维化形成的潜在靶点。
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