Guaragna Annalisa, Amoresano Angela, Pinto Vittoria, Monti Gianluca, Mastrobuoni Guido, Marino Gennaro, Palumbo Giovanni
Dipartimento di Chimica Organica e Biochimica Università di Napoli Federico II Via Cinthia, 4 I-80126 Napoli, Italy.
Bioconjug Chem. 2008 May;19(5):1095-104. doi: 10.1021/bc800010b. Epub 2008 Apr 12.
During recent years, quantitative proteome profiling has taken advantage of incorporating the traditional stable isotope dilution analysis into global scale or discovery-based proteomic experiments that use mass spectrometers as detectors to allow the pairwise study of differently expressed proteins. Quantitative protein analysis by means of the isotope-coded affinity tag (ICAT) method and tandem mass spectrometry (MS) enables the pairwise comparison of protein expression levels in biological samples. Herein, a modified ICAT reagent, named BAA-ICAT (beta-alanine-arm-ICAT) in which the polyether linker is replaced by a more water-soluble polyamide one, was investigated.
近年来，定量蛋白质组分析利用将传统的稳定同位素稀释分析纳入以质谱仪作为检测器的全球规模或基于发现的蛋白质组学实验中，从而能够对差异表达的蛋白质进行成对研究。通过同位素编码亲和标签（ICAT）方法和串联质谱（MS）进行定量蛋白质分析，能够对生物样品中的蛋白质表达水平进行成对比较。在此，研究了一种改良的ICAT试剂，名为BAA-ICAT（β-丙氨酸臂-ICAT），其中聚醚连接体被更具水溶性的聚酰胺连接体所取代。
