Division of Chemical Biology and Biotechnology, School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
Anal Chim Acta. 2011 Sep 30;702(2):149-59. doi: 10.1016/j.aca.2011.06.045. Epub 2011 Jun 30.
In terms of proteomic research in the 21st century, the realm of virology is still regarded as an enormous challenge mainly brought by three aspects, namely, studying on the complex proteome of the virus with unexpected variations, developing more accurate analytical techniques as well as understanding viral pathogenesis and virus-host interaction dynamics. Progresses in these areas will be helpful to vaccine design and antiviral drugs discovery. Mass spectrometry based proteomics have shown exceptional display of capabilities, not only precisely identifying viral and cellular proteins that are functionally, structurally, and dynamically changed upon virus infection, but also enabling us to detect important pathway proteins. In addition, many isolation and purification techniques and quantitative strategies in conjunction with MS can significantly improve the sensitivity of mass spectrometry for detecting low-abundant proteins, replenishing the stock of virus proteome and enlarging the protein-protein interaction maps. Nevertheless, only a small proportion of the infectious viruses in both of animal and plant have been studied using this approach. As more virus and host genomes are being sequenced, MS-based proteomics is becoming an indispensable tool for virology. In this paper, we provide a brief review of the current technologies and their applications in studying selected viruses and hosts.
在 21 世纪的蛋白质组学研究中，病毒学领域仍然被认为是一个巨大的挑战，主要由三个方面带来，即研究具有意外变异的病毒复杂蛋白质组、开发更准确的分析技术以及了解病毒发病机制和病毒-宿主相互作用动态。这些领域的进展将有助于疫苗设计和抗病毒药物的发现。基于质谱的蛋白质组学显示出了非凡的能力，不仅可以精确识别病毒感染后功能、结构和动态变化的病毒和细胞蛋白，还可以使我们能够检测到重要的途径蛋白。此外，许多与 MS 结合使用的分离和纯化技术和定量策略可以显著提高质谱检测低丰度蛋白质的灵敏度，补充病毒蛋白质组的库存并扩大蛋白质-蛋白质相互作用图谱。然而，只有一小部分动物和植物中的传染性病毒已经使用这种方法进行了研究。随着越来越多的病毒和宿主基因组被测序，基于 MS 的蛋白质组学正在成为病毒学不可或缺的工具。本文简要综述了目前的技术及其在研究选定病毒和宿主中的应用。
