Lasta Samar, Fajloun Ziad, Darbon Hervé, Mansuelle Pascal, Andreotti Nicolas, Sabatier Jean-Marc, Boudabous Abdellatif, Sampieri François
Laboratoire de Biochimie, CNRS FRE 2738, Faculté de Médecine Nord, Bd Pierre Dramard, 13916 Marseille Cedex 20, France.
J Antibiot (Tokyo). 2008 Feb;61(2):89-93. doi: 10.1038/ja.2008.116.
Bacteriocin J46 is a 27-residue polypeptide produced by Lactococcus lactis subsp. cremoris J46 in fermented milk. The natural form of J46 (nJ46) exhibits a broad antimicrobial spectrum. Herein, we produced the synthetic form of J46 (sJ46) by solid-phase chemical synthesis. The biochemical and physico-chemical properties of sJ46, as well as its antimicrobial activity, were found to be identical to those of its natural counterpart nJ46. It showed a potent antimicrobial activity against both lactic acid bacteria and other Gram-positive microorganisms. (1)H-NMR conformational analysis of sJ46 indicates that it adopts a flexible random coil structure.
细菌素J46是一种由乳酸乳球菌乳脂亚种J46在发酵乳中产生的27个氨基酸残基的多肽。J46的天然形式（nJ46）具有广泛的抗菌谱。在此，我们通过固相化学合成制备了J46的合成形式（sJ46）。发现sJ46的生化和物理化学性质及其抗菌活性与其天然对应物nJ46相同。它对乳酸菌和其他革兰氏阳性微生物均表现出强大的抗菌活性。sJ46的（1）H-NMR构象分析表明它采用灵活的无规卷曲结构。
