Stoianova L G, Egorov N S, Fedorova G B, Katrukha G S, Netrusov A I
Prikl Biokhim Mikrobiol. 2007 Nov-Dec;43(6):677-84.
Bacteriocins formed by four strains of Lactococcus lactis subsp. lactis have been studied and compared: 729 (a natural strain isolated from milk), 1605 (a mutant of strain 729), F-116 (a recombinant obtained by fusing of protoplasts of the two related strain 729 and 1605), and a nisin-forming strain obtained by adaptive selection at Moscow State University. Antimicrobial activity studies revealed differences between the strains in the effects on individual groups of microorganisms; the activities of the strains were also distinct from that of Nisaplin (a commercial preparation of the bacteriocin nisin). Methods for isolation and purification of bacteriocins have been developed, making it possible to obtain individual components of antibiotic complexes as chromatographically pure preparations. Bacteriocins formed by the strains of Lactococcus lactis subsp. lactis have been identified and differences in their biological and physicochemical properties, established. A novel potent broad-spectrum antibiotic substance distinct from nisin has been isolated from the recombinant strain F-116.
729（从牛奶中分离出的天然菌株）、1605（729菌株的突变体）、F - 116（通过融合两个相关菌株729和1605的原生质体获得的重组体），以及莫斯科国立大学通过适应性选择获得的产乳链菌肽菌株。抗菌活性研究揭示了这些菌株对不同微生物群体的影响存在差异；这些菌株的活性也与Nisaplin（细菌素乳链菌肽的商业制剂）不同。已经开发出分离和纯化细菌素的方法，从而能够获得作为色谱纯制剂的抗生素复合物的各个组分。已鉴定出乳酸乳球菌乳酸亚种菌株所产生的细菌素，并确定了它们在生物学和物理化学性质上的差异。从重组菌株F - 116中分离出了一种不同于乳链菌肽的新型强效广谱抗生素物质。
