Amano Nobuyuki, Takai Shinji, Jin Denan, Ueda Koichi, Miyazaki Mizuo
Department of Pharmacology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki City, Osaka, 569-8686, Japan.
Lasers Med Sci. 2009 Mar;24(2):223-9. doi: 10.1007/s10103-008-0558-y. Epub 2008 Apr 12.
The relationships between mast cell-derived chymase, angiotensin II, and extracellular matrix production in the skin after intense pulsed light (IPL) were clarified in hamsters. Dorsal areas of the hamsters were irradiated once or twice a week by IPL. The index of extracellular matrix production in the skin was defined as the depth stained with Azan-Mallory stain from the epidermis to the dermis at the point of maximum thickness. The index had significantly increased 7 days after IPL irradiation in sections treated once or twice with IPL compared with that of untreated control sections. The numbers of mast cells, chymase-positive cells, and angiotensin II-positive cells had also significantly increased in IPL-irradiated areas. Significant increases in chymase and angiotensin II activities were observed in the extracts obtained from IPL-irradiated skin. Mast cell-derived chymase may be involved via angiotensin II formation in the dermal extracellular matrix production that occurs after IPL irradiation.
在仓鼠中阐明了强脉冲光（IPL）照射后皮肤中肥大细胞衍生的糜酶、血管紧张素II与细胞外基质产生之间的关系。每周对仓鼠背部区域进行一次或两次IPL照射。皮肤中细胞外基质产生的指标定义为在最大厚度处从表皮到真皮用偶氮-马洛里染色法染色的深度。与未处理的对照切片相比，用IPL处理一次或两次的切片在IPL照射7天后该指标显著增加。在IPL照射区域，肥大细胞、糜酶阳性细胞和血管紧张素II阳性细胞的数量也显著增加。从IPL照射的皮肤提取物中观察到糜酶和血管紧张素II活性显著增加。肥大细胞衍生的糜酶可能通过血管紧张素II的形成参与IPL照射后发生的真皮细胞外基质产生。
