衣藻叶绿体atpB基因的3'茎环结构在体内调节mRNA积累。
A 3' stem/loop structure of the Chlamydomonas chloroplast atpB gene regulates mRNA accumulation in vivo.
作者信息
Stern D B, Radwanski E R, Kindle K L
机构信息
Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853.
出版信息
Plant Cell. 1991 Mar;3(3):285-97. doi: 10.1105/tpc.3.3.285.
Abstract
The Chlamydomonas reinhardtii chloroplast atpB mRNA contains sequences at its 3' end that can form a complex stem/loop structure. Deletions of part or all of this sequence in transformed C. reinhardtii cells led to decreased atpB mRNA accumulation, whereas transcription rates were unaffected. The reduction of mRNA to 20% to 35% of wild-type levels in transformants without 3' stem/loops was correlated with the accumulation of atpB mRNA that was heterogeneous in size. These results indicated that RNA secondary structures function both in mRNA stabilization and in 3' end formation in C. reinhardtii chloroplasts. Furthermore, deletion of the stem/loop resulted in a decrease in the steady-state level of the ATPase beta-subunit to approximately 60% of wild-type levels, suggesting that translational and/or post-translational mechanisms may influence the steady-state level of the atpB gene product.
摘要
莱茵衣藻叶绿体的atpB mRNA在其3'端含有可形成复杂茎环结构的序列。在转化的莱茵衣藻细胞中缺失该序列的部分或全部会导致atpB mRNA积累减少，而转录速率不受影响。在没有3'茎环的转化体中，mRNA降至野生型水平的20%至35%，这与大小各异的atpB mRNA积累相关。这些结果表明，RNA二级结构在莱茵衣藻叶绿体的mRNA稳定化和3'端形成中均发挥作用。此外，茎环的缺失导致ATP酶β亚基的稳态水平降至野生型水平的约60%，这表明翻译和/或翻译后机制可能会影响atpB基因产物的稳态水平。
相似文献
1
A 3' stem/loop structure of the Chlamydomonas chloroplast atpB gene regulates mRNA accumulation in vivo.衣藻叶绿体atpB基因的3'茎环结构在体内调节mRNA积累。
Plant Cell. 1991 Mar;3(3):285-97. doi: 10.1105/tpc.3.3.285.
2
The sequence and structure of the 3'-untranslated regions of chloroplast transcripts are important determinants of mRNA accumulation and stability.叶绿体转录本3'非翻译区的序列和结构是mRNA积累和稳定性的重要决定因素。
Plant Mol Biol. 1998 Jan;36(2):307-14. doi: 10.1023/a:1005943701253.
3
The chloroplast gene cluster containing psbF, psbL, petG and rps3 is conserved in Chlamydomonas.包含psbF、psbL、petG和rps3的叶绿体基因簇在衣藻中是保守的。
Curr Genet. 1994 Dec;27(1):54-61. doi: 10.1007/BF00326579.
4
The 3' untranslated regions of chloroplast genes in Chlamydomonas reinhardtii do not serve as efficient transcriptional terminators.莱茵衣藻叶绿体基因的3'非翻译区不能作为有效的转录终止子。
Mol Gen Genet. 1996 Oct 28;252(6):676-83. doi: 10.1007/BF02173973.
5
3'-Processed mRNA is preferentially translated in Chlamydomonas reinhardtii chloroplasts.3' 加工的信使核糖核酸（mRNA）在莱茵衣藻叶绿体中优先被翻译。
Mol Cell Biol. 1998 Aug;18(8):4605-11. doi: 10.1128/MCB.18.8.4605.
6
Altering the 3 UTR endonucleolytic cleavage site of a Chlamydomonas chloroplast mRNA affects 3-end maturation in vitro but not in vivo.改变衣藻叶绿体mRNA的3'非翻译区（3'UTR）核酸内切酶切割位点会影响体外3'末端成熟，但不影响体内3'末端成熟。
Plant Mol Biol. 1999 Jul;40(4):679-86. doi: 10.1023/a:1006252201661.
7
Antisense transcript and RNA processing alterations suppress instability of polyadenylated mRNA in chlamydomonas chloroplasts.反义转录本和RNA加工改变抑制衣藻叶绿体中多聚腺苷酸化mRNA的不稳定性。
Plant Cell. 2004 Nov;16(11):2849-69. doi: 10.1105/tpc.104.026203. Epub 2004 Oct 14.
8
3'end maturation of the Chlamydomonas reinhardtii chloroplast atpB mRNA is a two-step process.莱茵衣藻叶绿体atpB mRNA的3'末端成熟是一个两步过程。
Mol Cell Biol. 1993 Apr;13(4):2277-85. doi: 10.1128/mcb.13.4.2277-2285.1993.
9
10
Transcriptional analysis of endogenous and foreign genes in chloroplast transformants of Chlamydomonas.衣藻叶绿体转化体中内源基因和外源基因的转录分析。
Plant Cell. 1990 Nov;2(11):1059-70. doi: 10.1105/tpc.2.11.1059.
引用本文的文献
1
Ribosome-controlled transcription termination is essential for the production of antibiotic microcin C.核糖体控制的转录终止对于抗生素小菌素C的产生至关重要。
Nucleic Acids Res. 2014 Oct 29;42(19):11891-902. doi: 10.1093/nar/gku880. Epub 2014 Oct 1.
2
Genetic engineering of thylakoid protein complexes by chloroplast transformation in Chlamydomonas reinhardtii.通过叶绿体转化在莱茵衣藻中对类囊体蛋白复合物进行遗传工程改造。
Photosynth Res. 1995 May;44(1-2):191-205. doi: 10.1007/BF00018309.
3
A 43 kD light-regulated chloroplast RNA-binding protein interacts with the psbA 5' non-translated leader RNA.一种 43kD 的光调控叶绿体 RNA 结合蛋白与 psbA 5'非翻译前导 RNA 相互作用。
Photosynth Res. 1995 Nov;46(1-2):235-48. doi: 10.1007/BF00020436.
4
Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives.莱茵衣藻作为生产重组蛋白的可行平台：现状与展望。
Plant Cell Rep. 2012 Mar;31(3):479-94. doi: 10.1007/s00299-011-1186-8. Epub 2011 Nov 12.
5
The microalga Chlamydomonas reinhardtii as a platform for the production of human protein therapeutics.莱茵衣藻这种微藻作为生产人类蛋白质治疗药物的平台。
Bioeng Bugs. 2011 Jan-Feb;2(1):50-4. doi: 10.4161/bbug.2.1.13423.
6
Evolution of the rpoB-psbZ region in fern plastid genomes: notable structural rearrangements and highly variable intergenic spacers.蕨类植物质体基因组 rpoB-psbZ 区的进化：显著的结构重排和高度可变的基因间隔区。
BMC Plant Biol. 2011 Apr 13;11:64. doi: 10.1186/1471-2229-11-64.
7
The effect of different 3' untranslated regions on the accumulation and stability of transcripts of a gfp transgene in chloroplasts of transplastomic tobacco.不同 3'非翻译区对 GFP 转基因在转基因烟草叶绿体中积累和稳定性的影响。
Plant Mol Biol. 2011 Jul;76(3-5):385-96. doi: 10.1007/s11103-010-9689-1. Epub 2010 Sep 22.
8
Distinct roles for the 5' and 3' untranslated regions in the degradation and accumulation of chloroplast tufA mRNA: identification of an early intermediate in the in vivo degradation pathway.叶绿体tufA mRNA降解与积累过程中5'和3'非翻译区的不同作用：体内降解途径中早期中间体的鉴定
Plant Mol Biol. 2007 Mar;63(5):689-702. doi: 10.1007/s11103-006-9117-8. Epub 2006 Dec 17.
9
The nucleus-encoded factor MCD4 participates in degradation of nonfunctional 3' UTR sequences generated by cleavage of pre-mRNA in Chlamydomonas chloroplasts.细胞核编码因子MCD4参与衣藻叶绿体中前体mRNA切割产生的无功能3'UTR序列的降解。
Mol Genet Genomics. 2007 Mar;277(3):329-40. doi: 10.1007/s00438-006-0192-y. Epub 2006 Dec 7.
10
Regulatory sequences of orthologous petD chloroplast mRNAs are highly specific among Chlamydomonas species.直系同源petD叶绿体mRNA的调控序列在衣藻物种中具有高度特异性。
Plant Mol Biol. 2006 Feb;60(3):405-22. doi: 10.1007/s11103-005-4477-z.
本文引用的文献
1
Relationship between Gene Dosage and Gene Expression in the Chloroplast of Chlamydomonas reinhardtii.莱茵衣藻叶绿体中基因剂量与基因表达的关系
Plant Physiol. 1989 Oct;91(2):648-55. doi: 10.1104/pp.91.2.648.
2
Perturbation of chloroplast DNA amounts and chloroplast gene transmission in Chlamydomonas reinhardtii by 5-fluorodeoxyuridine.5-氟脱氧尿苷对莱茵衣藻叶绿体DNA含量和叶绿体基因传递的扰动
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4552-6. doi: 10.1073/pnas.74.10.4552.
3
Transcription and RNA stability are important determinants of higher plant chloroplast RNA levels.转录和 RNA 稳定性是高等植物叶绿体 RNA 水平的重要决定因素。
EMBO J. 1987 Jun;6(6):1571-9. doi: 10.1002/j.1460-2075.1987.tb02402.x.
4
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
5
Control of plastid gene expression during development: the limited role of transcriptional regulation.发育过程中质体基因表达的调控：转录调控的有限作用。
Cell. 1987 May 8;49(3):379-87. doi: 10.1016/0092-8674(87)90290-x.
6
Transcription termination and the regulation of gene expression.转录终止与基因表达调控。
Annu Rev Biochem. 1986;55:339-72. doi: 10.1146/annurev.bi.55.070186.002011.
7
A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.GM-CSF信使核糖核酸3'非翻译区的一段保守AU序列介导选择性信使核糖核酸降解。
Cell. 1986 Aug 29;46(5):659-67. doi: 10.1016/0092-8674(86)90341-7.
8
The stem-loop structure at the 3' end of histone mRNA is necessary and sufficient for regulation of histone mRNA stability.组蛋白mRNA 3'端的茎环结构对于组蛋白mRNA稳定性的调控是必需且充分的。
Mol Cell Biol. 1987 Dec;7(12):4557-9. doi: 10.1128/mcb.7.12.4557-4559.1987.
9
Differential mRNA stability to reticulocyte ribonucleases correlates with 3' non-coding (U)nA sequences.对网织红细胞核糖核酸酶的差异mRNA稳定性与3'非编码（U）nA序列相关。
Eur J Biochem. 1988 Mar 1;172(2):333-40. doi: 10.1111/j.1432-1033.1988.tb13891.x.
10
Mechanisms of mRNA decay in bacteria: a perspective.细菌中mRNA降解的机制：一个视角。
Gene. 1988 Dec 10;72(1-2):15-23. doi: 10.1016/0378-1119(88)90123-0.
Zotero 插件