Stern D B, Radwanski E R, Kindle K L
Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853.
Plant Cell. 1991 Mar;3(3):285-97. doi: 10.1105/tpc.3.3.285.
The Chlamydomonas reinhardtii chloroplast atpB mRNA contains sequences at its 3' end that can form a complex stem/loop structure. Deletions of part or all of this sequence in transformed C. reinhardtii cells led to decreased atpB mRNA accumulation, whereas transcription rates were unaffected. The reduction of mRNA to 20% to 35% of wild-type levels in transformants without 3' stem/loops was correlated with the accumulation of atpB mRNA that was heterogeneous in size. These results indicated that RNA secondary structures function both in mRNA stabilization and in 3' end formation in C. reinhardtii chloroplasts. Furthermore, deletion of the stem/loop resulted in a decrease in the steady-state level of the ATPase beta-subunit to approximately 60% of wild-type levels, suggesting that translational and/or post-translational mechanisms may influence the steady-state level of the atpB gene product.
莱茵衣藻叶绿体的atpB mRNA在其3'端含有可形成复杂茎环结构的序列。在转化的莱茵衣藻细胞中缺失该序列的部分或全部会导致atpB mRNA积累减少，而转录速率不受影响。在没有3'茎环的转化体中，mRNA降至野生型水平的20%至35%，这与大小各异的atpB mRNA积累相关。这些结果表明，RNA二级结构在莱茵衣藻叶绿体的mRNA稳定化和3'端形成中均发挥作用。此外，茎环的缺失导致ATP酶β亚基的稳态水平降至野生型水平的约60%，这表明翻译和/或翻译后机制可能会影响atpB基因产物的稳态水平。
