CYP1A1 2B和4基因多态性与墨西哥患者的肺癌易感性相关。

CYP1A1 2B and 4 polymorphisms are associated with lung cancer susceptibility in Mexican patients.

作者信息

Gallegos-Arreola M P, Figuera-Villanueva L E, Troyo-Sanroman R, Morgán-Villela G, Puebla-Pérez A M, Flores-Marquez M R, Zúniga-González G M

机构信息

Molecular Genetics Laboratory, Division of Molecular Medicine, Centro de Investigación Biomédica de Occidente, Guadalajara, Jalisco - Mexico.

出版信息

Int J Biol Markers. 2008 Jan-Mar;23(1):24-30.

PMID:18409147

Abstract

BACKGROUND

CYP1A1 is a gene involved in the high aryl hydrocarbon hydroxylase -inducible phenotype, which is a genetically-determined variation among individuals that has been associated with lung cancer risk. More specifically, CYP1A1 *2B and *4 polymorphisms have been associated with high susceptibility to lung cancer among cigarette smokers.

MATERIALS AND METHODS

DNA was obtained from blood samples and we studied by PCR-RFLP the distribution of CYP1A1 *2B (n=248) and *4 (n=222) polymorphisms in healthy controls and 222 lung cancer patients from a Mexican population.

RESULTS

Comparisons between groups showed an increased risk for lung cancer patients of *2B/*2B (18%; OR 7.6; 95% CI 3.0-19.2) and *4/ *4 genotypes (15%; OR 11.45; 95% CI 2.19-59.85) compared to the control group (1% for *2B/ *2B and 4.4% for *4/ *4). A significant association between lung cancer and homozygous *2B/ *2B passive smokers and *4/*4 ever (cigarettes) and passive smokers was also observed (p<0.05). Multivariate analysis revealed an increased risk for the *2B/*2B genotype (OR 6.83), as well as for *4/*4 (OR 28.8).

CONCLUSION

The results of the study indicate a significant association between *2B/*2B and *4/*4 genotypes and the risk of developing lung cancer among Mexicans.

摘要

背景

CYP1A1是一种与高芳烃羟化酶诱导表型相关的基因，该表型是个体间的一种基因决定变异，与肺癌风险相关。更具体地说，CYP1A1 2B和4多态性与吸烟者患肺癌的高易感性相关。

材料与方法

从血样中获取DNA，我们通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）研究了来自墨西哥人群的222例肺癌患者和健康对照者中CYP1A1 2B（n = 248）和4（n = 222）多态性的分布。

结果

组间比较显示，与对照组（*2B/*2B为1%，*4/4为4.4%）相比，肺癌患者中2B/2B（18%；比值比7.6；95%置信区间3.0 - 19.2）和4/4基因型（15%；比值比11.45；95%置信区间2.19 - 59.85）的风险增加。还观察到肺癌与纯合子2B/2B被动吸烟者以及4/4曾经（吸烟）和被动吸烟者之间存在显著关联（p < 0.05）。多变量分析显示2B/2B基因型（比值比6.83）以及4/*4（比值比28.8）的风险增加。