从花椰菜（ Brassica oleracea L.）的肽和 cDNA 序列推导出细胞色素 b(5)的一级结构。
Primary Structure of Cytochrome b(5) from Cauliflower (Brassica oleracea L.) Deduced from Peptide and cDNA Sequences.
机构信息
Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312.
出版信息
Plant Physiol. 1992 Jul;99(3):1254-7. doi: 10.1104/pp.99.3.1254.
Abstract
Cytochrome b(5) is a microsomal protein that functions as an intermediate electron donor in fatty acid desaturation and other oxidation/reduction reactions. cDNA clones were isolated from cauliflower (Brassica oleracea L.) by using oligonucleotides based on the partial amino acid sequence of the protein. The deduced amino acid sequence of the polypeptide exhibited approximately 30% sequence identity with the homologous protein from vertebrates.
摘要
细胞色素 b(5)是一种微粒体蛋白，作为脂肪酸去饱和作用和其他氧化/还原反应的中间电子供体。通过使用基于该蛋白部分氨基酸序列的寡核苷酸，从花椰菜（甘蓝型油菜）中分离出 cDNA 克隆。推断的多肽氨基酸序列与脊椎动物的同源蛋白具有约 30%的序列同一性。
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