Gasser C. S., Budelier K. A., Smith A. G., Shah D. M., Fraley R. T.
Plant Molecular Biology, Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, Missouri 63198.
Plant Cell. 1989 Jan;1(1):15-24. doi: 10.1105/tpc.1.1.15.
We have used a differential plaque hybridization screening procedure to isolate cDNA clones for genes that show elevated or exclusive expression in tomato pistils. Clones that showed maximal expression in immature pistils (premeiotic to early meiosis) and mature pistils (at anthesis) were isolated. Of nine clones that were characterized, four were found also to express at some stage of anther development. In situ hybridization experiments showed that expression of the genes we have identified is very tightly regulated both spatially and temporally within the pistil. One gene was identified that is expressed in the pistil only in the transmitting tissue of the style. A second gene was found to express exclusively in two to three cell layers of the ovules for a period of less than eight days.
我们采用了差异菌斑杂交筛选程序,以分离在番茄雌蕊中表达升高或特异性表达的基因的cDNA克隆。分离出了在未成熟雌蕊(减数分裂前至减数分裂早期)和成熟雌蕊(开花期)中显示最大表达的克隆。在已鉴定的9个克隆中,发现有4个在花药发育的某个阶段也有表达。原位杂交实验表明,我们鉴定出的基因在雌蕊内的表达在空间和时间上都受到非常严格的调控。鉴定出一个基因,其仅在花柱的传递组织中的雌蕊中表达。还发现第二个基因在胚珠的两到三层细胞中特异性表达,持续时间不到八天。