前列腺癌中12S/MT-RNR1、MT-CO2/COX2和MT-ATP6线粒体基因的表达改变。

Altered expression of 12S/MT-RNR1, MT-CO2/COX2, and MT-ATP6 mitochondrial genes in prostate cancer.

作者信息

Abril Jesús, de Heredia Miguel López, González Laura, Clèries Ramon, Nadal Marga, Condom Enric, Aguiló Fernando, Gómez-Zaera Montserrat, Nunes Virginia

机构信息

Centre de Genètica Mèdica i Molecular-IDIBELL, Hospital Duran i Reynals, L'Hospitalet de Llobregat, Barcelona, Spain.

出版信息

Prostate. 2008 Jul 1;68(10):1086-96. doi: 10.1002/pros.20771.

DOI:10.1002/pros.20771

PMID:18409190

Abstract

BACKGROUND

Prostate cancer is one of the commonest cancers worldwide and is responsible for nearly 6% of all male cancer deaths. Despite this relevance, the mechanisms involved in the development and progression of this malignancy remain unknown. The involvement of polypeptides of the mitochondrial respiratory chain, the Krebs cycle and the glutathione antioxidant system in this type of cancer has been previously described, although no publication has focused on the expression of mitochondrial genes in the prostate of PCa patients.

METHODS

We have determined by reverse transcription-quantitative PCR (RT-qPCR) the relative amount of the transcripts of eight mitochondrial genes (MT-ND2, MT-ND4, MT-ND6, MT-CYB, 12S/MT-RNR1, 16S/MT-RNR2, MT-CO2/COX2, MT-ATP6), and four nuclear genes (COX11, GSR, CS, ACO2), all of them key players in the normal metabolism of mitochondria. Additionally we analyzed the expression of Cyclophilin A (PPIA).

RESULTS

We observed differential expression of mitochondrial 12S/MT-RNR1, MT-CO2/COX2, and MT-ATP6 transcripts in tumor samples when compared to their paired normal samples.

CONCLUSIONS

The amount of mitochondrial 12S/MT-RNR1, MT-CO2/COX2, and MT-ATP6 transcripts is significantly decreased in tumor samples when compared to their paired normal sample, suggesting that mitochondrial gene expression is altered in PCa.

摘要

背景

前列腺癌是全球最常见的癌症之一，占男性癌症死亡总数的近6%。尽管具有这种相关性，但这种恶性肿瘤发生和发展所涉及的机制仍然未知。此前已有文献描述了线粒体呼吸链、三羧酸循环和谷胱甘肽抗氧化系统的多肽在这类癌症中的作用，不过尚无关于前列腺癌患者前列腺中线粒体基因表达的报道。

方法

我们通过逆转录定量聚合酶链反应（RT-qPCR）测定了八个线粒体基因（MT-ND2、MT-ND4、MT-ND6、MT-CYB、12S/MT-RNR1、16S/MT-RNR2、MT-CO2/COX2、MT-ATP6）以及四个核基因（COX11、GSR、CS、ACO2）的转录本相对含量，这些基因都是线粒体正常代谢中的关键因子。此外，我们还分析了亲环蛋白A（PPIA）的表达。