Kedziora Hanna, Florek Ewa, Piekoszewski Wojciech, Breborowicz Grzegorz H, Anholcer Andrzej, Gomółka Ewa, Kulza Maksymilian, Stanek Artur
Laboratorium Badań Srodowiskowych, Katedra i Zakład Toksykologii, Uniwersytet Medyczny im. Karola Marcinkowskiego w Poznaniu.
Przegl Lek. 2007;64(10):740-6.
To assess the exposure to tobacco smoke constituents, the biomarkers are used. In most studies conducted among active smokers, the cotinine is usually utilised as such a biomarker. The aim of this study was to estimate an application of determining two nicotine metabolites, cotinine and trans-3'-hydroxycotinine (3-HC) in order to assess cigarette smoking among pregnant women. There were 25 patients (15 smokers, 10 non-smokers) admitted to Delivery Ward in Gynecology and Obstetrics Clinical Hospital of the University of Medical Sciences in Poznan. The free and total form of cotinine and trans-3'-hydroxycotinine in urine were determined by the means of high performance liquid chromatography with spectrometry detection. The results of this studies indicated that in urine both cotinine and trans-3'-hydroxycotinine are conjugated with glucoronide acid in the high degree. The linear correlation between free and conjugated form of cotinine and whole cotinine concentration indicates the possibility of application of all three of them as biomarkers of tobacco smoke exposure. The high conjugation of trans-3'-hydroxycotinine with glucuronic acid (over 80 %), and high correlation between glucuronide and total trans-3'-hydroxycotinine concentration proves necessity of having the urine samples hydrolysed or determining the 3'-hydroxycotinine glucoronide, when application of 3-HC as a biomarker of tobacco smoke exposure is concerned. Our studies confirm other authors' observations that division of 3'-hydroxycotinine and cotinine concentrations might be used as a predictor of differences in cotinine metabolite ratio (polimorphism). The studies shown that using analytical equipment, nowadays available in the most laboratories (HPLC), it is possible to determined two biomarkers which are very useful and give various information, no matter whether they are conjugated with glucuronic acid, or not.
为评估烟草烟雾成分的暴露情况，需使用生物标志物。在大多数针对主动吸烟者开展的研究中，可替宁通常被用作此类生物标志物。本研究的目的是评估测定两种尼古丁代谢物——可替宁和反式-3'-羟基可替宁（3-HC）在评估孕妇吸烟情况方面的应用。有25名患者（15名吸烟者，10名非吸烟者）入住了波兹南医科大学妇产科临床医院的产房。采用高效液相色谱-光谱检测法测定尿液中可替宁和反式-3'-羟基可替宁的游离形式和总形式。本研究结果表明，尿液中的可替宁和反式-3'-羟基可替宁都与葡萄糖醛酸高度结合。可替宁游离形式与结合形式以及总可替宁浓度之间的线性相关性表明，它们三者都有可能作为烟草烟雾暴露的生物标志物。反式-3'-羟基可替宁与葡萄糖醛酸的结合率很高（超过80%），且葡萄糖醛酸与总反式-3'-羟基可替宁浓度之间的高度相关性证明，在将3-HC用作烟草烟雾暴露生物标志物时，有必要对尿液样本进行水解或测定3'-羟基可替宁葡萄糖醛酸。我们的研究证实了其他作者的观察结果，即3'-羟基可替宁和可替宁浓度的比值可作为可替宁代谢物比率（多态性）差异的预测指标。研究表明，使用目前大多数实验室都具备的分析设备（高效液相色谱仪），可以测定两种非常有用且能提供各种信息的生物标志物，无论它们是否与葡萄糖醛酸结合。
