[The intranatal analysis of trans-3'-hydroxycotinine concentrations in serum of mother and neonate exposed to tobacco smoke].

The smoking habit is widely spread between pregnant women too. Tobacco smoke components have negative influence on infant's development. In human trans-3'-hydroxycotinine is besides cotinine, one of the main nicotine metabolites. The research covered 17 patients with physiological pregnancy, which ended with delivery through natural passages at term. All women were active smokers as we revealed before according to their serum cotinine concentrations. Assessment of cotinine and trans-3'-hydroxycotinine concentrations has been done by the use of very sensitive liquid chromatographic techniques in the mothers venous blood samples and in the mixed umbilical cord blood of the newborns. Obtained results of the research have been statistically analysed using the STATISTICA package. Significance level p < 0.05 has been adopted as statistically relevant. In conclusion, trans-3'-hydroxycotinine concentrations were significantly lower in the neonates then in their smoking mothers and there was strong positive linear relationship between maternal and neonatal trans-3'-hydroxycotinine concentrations. The value of linear correlation coefficient was 0,7 with a p-value of 0.05.