Mindlin S Z, Soina V S, Ptrova M A, Gorlenko Zh M
Genetika. 2008 Jan;44(1):36-44.
A collection of bacterial antibiotic resistance strains isolated from arctic permafrost subsoil sediments of various age and genesis was created. The collection included approximately 100 strains of Gram-positive (Firmicutes, Arthrobacter) and Gram-negative bacteria (Bacteroidetes, gamma-Proteobacteria, and alpha-Proteobacteria) resistant to aminoglycoside antibiotics (gentamycin, kanamycin, and streptomycin), chloramphenicol and tetracycline. Antibiotic resistance spectra were shown to differ in Gram-positive and Gram-negative bacteria. Multidrug resistance strains were found for the first time in ancient bacteria. In studies of the molecular nature of determinants for streptomycin resistance, determinants of the two types were detected: strA-strB genes coding for aminoglycoside phosphotransferases and genes aadA encoding aminoglycoside adenylyltransferases. These genes proved to be highly homologous to those of contemporary bacteria.
构建了一个从不同年代和成因的北极永久冻土亚表层沉积物中分离出的细菌抗生素抗性菌株库。该菌株库包含约100株对氨基糖苷类抗生素（庆大霉素、卡那霉素和链霉素）、氯霉素和四环素具有抗性的革兰氏阳性菌（厚壁菌门、节杆菌属）和革兰氏阴性菌（拟杆菌门、γ-变形菌纲和α-变形菌纲）。结果表明，革兰氏阳性菌和革兰氏阴性菌的抗生素抗性谱有所不同。在古老细菌中首次发现了多重耐药菌株。在对链霉素抗性决定因素的分子性质研究中，检测到了两种类型的决定因素：编码氨基糖苷磷酸转移酶的strA-strB基因和编码氨基糖苷腺苷酸转移酶的aadA基因。这些基因被证明与当代细菌的基因高度同源。
