Tranfo G, Pigini D, Federici G, Bernardini S, Perricone C, Perricone R
ISPESL, Centro Ricerche di Monte Porzio Catone.
G Ital Med Lav Ergon. 2007 Jul-Sep;29(3 Suppl):520-2.
Immunological methods for the study of the plasma complement system have been standardized in order to be good and reproducible indicators of some biological effects of the substances under study in in vitro experiments. The substances tested were not capable of interfering within 10 times the possible hypothetical plasma concentration reached in vivo with the function of the different reagents used in the study of complement. Five substances (Skin-ACGIH) have been studied for their effects on the complement system in vitro; four of them could be fully studied (allylic alcohol, cyclohexanone, phenol, dimethylacetamide). After this deep insight we can conclude that: 1. These substances are capable of interfering with the immune response through their complement activating capacity 2. These substances, throughout complement activation, can induce inflammation and reduction of important defensive functions that are complement mediated. 3. The results obtained encourage to study the complement system and especially CH50 in workers exposed to the selected substances in order to verify the possibility to enclose this test in the medical surveillance program.
为了成为体外实验中所研究物质某些生物学效应的良好且可重复的指标，用于研究血浆补体系统的免疫学方法已得到标准化。所测试的物质在体内可能达到的假设血浆浓度的10倍范围内，不会干扰补体研究中使用的不同试剂的功能。已研究了五种物质（皮肤 - 美国政府工业卫生学家会议阈限值）对体外补体系统的影响；其中四种物质（烯丙醇、环己酮、苯酚、二甲基乙酰胺）可进行全面研究。经过深入研究，我们可以得出以下结论：1. 这些物质能够通过其补体激活能力干扰免疫反应。2. 这些物质在补体激活过程中，可诱导炎症并降低补体介导的重要防御功能。3. 所获得的结果鼓励对接触所选物质的工人的补体系统，尤其是总补体活性（CH50）进行研究，以验证将该测试纳入医学监测计划的可能性。
