Cavallo Delia, Ursini Cinzia Lucia, Rondinone Bruna, Iavicoli Sergio
Department of Occupational Medicine, ISPESL-National Institute for Occupational Safety and Prevention, 00040 Monteporzio Catone, Rome, Italy.
Environ Mol Mutagen. 2009 Dec;50(9):781-90. doi: 10.1002/em.20501.
The objective of this study was to identify a sensitive and noninvasive biomarker of early genotoxic effects, for health risk assessment of workers exposed to mixtures of low doses of xenobiotics. We studied 30 workers exposed to antineoplastic drugs, 57 workers exposed to different mixtures of polycyclic aromatic hydrocarbons (PAHs) (41 airport workers and 16 paving workers) and 76 controls. Comet and micronucleus (MN) tests were performed on lymphocytes and exfoliated buccal cells. The MN assay on lymphocytes did not show significant differences between exposed and controls, while the MN assay on exfoliated buccal cells showed higher values in workers exposed to antineoplastics as compared with controls (0.85 vs. 0.48, P = 0.042). The comet assay on lymphocytes showed a higher comet percentage value (18.11 vs. 11.24 in controls, P = 0.001) and mean tail moment (TM) value (21.84 vs. 16.72 in controls, P = 0.003) in individuals exposed to PAHs as compared with controls; no significant differences were found in exposed to antineoplastics. The comet assay on exfoliated buccal cells did not show significant differences between exposed and control groups for comet percentages, whereas the TM value was higher in workers exposed to PAHs (55.1 vs. 32.31 for controls, P < 0.001). These results show that exfoliated buccal cells, obtained by a noninvasive procedure, represent robust target cells to assess the occupational exposure to inhalable mixture of chemicals at low doses. The comet assay seems to be suitable to promptly evaluate the genotoxic effects of PAHs mixtures that also contain volatile substances. The MN test is suitable to evaluate the effects of antineoplastics. Inc.
本研究的目的是确定一种敏感且无创的早期遗传毒性效应生物标志物，用于评估接触低剂量外源性化学物混合物的工人的健康风险。我们研究了30名接触抗肿瘤药物的工人、57名接触不同多环芳烃（PAH）混合物的工人（41名机场工人和16名铺路工人）以及76名对照者。对淋巴细胞和脱落的颊细胞进行了彗星试验和微核（MN）试验。淋巴细胞的MN试验在接触组和对照组之间未显示出显著差异，而脱落颊细胞的MN试验显示，与对照组相比，接触抗肿瘤药物的工人的MN值更高（0.85对0.48，P = 0.042）。与对照组相比，接触PAH的个体淋巴细胞的彗星试验显示彗星百分比值更高（对照组为11.24，接触组为18.11，P = 0.001），平均尾矩（TM）值更高（对照组为16.72，接触组为21.84，P = 0.003）；接触抗肿瘤药物的个体未发现显著差异。脱落颊细胞的彗星试验在接触组和对照组之间的彗星百分比方面未显示出显著差异，而接触PAH的工人的TM值更高（对照组为32.31，接触组为55.1，P < 0.001）。这些结果表明，通过无创程序获得的脱落颊细胞是评估低剂量可吸入化学物混合物职业暴露的可靠靶细胞。彗星试验似乎适合快速评估还含有挥发性物质的PAH混合物的遗传毒性效应。MN试验适合评估抗肿瘤药物的效应。公司
