Wang An-hui, Wang Ai-qin, Xu De-zhong, Men Ke, Yan Yong-ping, Zhang Jing-xia, Liu Yuan, Huang Xiao-feng, Wang Chun-mei
Department of Epidemiology, School of Preventive Medicine, Fourth Military Medical University, Xi'an 710033, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008 Feb;22(1):51-3.
To observe the changes of human trophoblast cells after infected with hepatitis B virus.
HBV positive serum was used to infect human trophoblast cells in vitro. HBsAg in cell culture medium were detected by ELISA method and HBV DNA in cell culture medium and cells were detected by PCR method. HBV fluorescence polymerase chain reaction diagnose kit were used to detect the HBV DNA concentration. Ultra structure of trophoblast cells were observed with transmission electron microscopy (TEM).
HBsAg could be detected in infection group by ELISA. Infection group cell culture medium and infection group cells were HBV DNA positive. HBV DNA concentrations in HBV infection cell culture medium in 0, 12, 36, 60, 84 h after extensively PBS washed were < 10(3), 3 x 10(4), 6 x 10(5), 5 x 10(5), 3 x 10(5) copies/mL. HBV infected trophoblast cells were found many forms of endosomes, some of which contents virus like particle.
HBV might take advantage of clathrin-mediated endocytosis to enter trophoblast cell, which might lead to cell infection or across the cell bar by transcytosis.
观察人滋养层细胞感染乙型肝炎病毒后的变化。
用乙肝病毒阳性血清体外感染人滋养层细胞。采用酶联免疫吸附测定法检测细胞培养基中的乙肝表面抗原，采用聚合酶链反应法检测细胞培养基和细胞中的乙肝病毒脱氧核糖核酸。使用乙肝病毒荧光聚合酶链反应诊断试剂盒检测乙肝病毒脱氧核糖核酸浓度。用透射电子显微镜观察滋养层细胞的超微结构。
酶联免疫吸附测定法可在感染组检测到乙肝表面抗原。感染组细胞培养基和感染组细胞的乙肝病毒脱氧核糖核酸呈阳性。经大量磷酸盐缓冲盐水洗涤后，在0、12、36、60、84小时时乙肝病毒感染细胞培养基中的乙肝病毒脱氧核糖核酸浓度分别为<10(3)、3×10(4)、6×10(5)、5×10(5)、3×10(5)拷贝/毫升。发现乙肝病毒感染的滋养层细胞有多种形式的内体，其中一些含有病毒样颗粒。
乙肝病毒可能利用网格蛋白介导的内吞作用进入滋养层细胞，这可能导致细胞感染或通过转胞吞作用穿过细胞屏障。
