Huang Tsui-Hsien, Liao Pao-Hsin, Li Han Yu, Ding Shinn Jyh, Yen Min, Kao Chia-Tze
Chung Shan Medical University Hospital, Institute of Stomatology, Institute of Oral Material Science, Taichung, Taiwan.
Angle Orthod. 2008 May;78(3):510-6. doi: 10.2319/053007-259.1.
To test the null hypothesis that the resin base and the resin hybrid glass ionomer base adhesives do not cause inflammation after contacting primary human gingival fibroblasts in vitro.
The resin base and resin hybrid glass ionomer base adhesives were used to treat human gingival fibroblasts to evaluate the survival rate using MTT colorimetric assay to detect the level of cyclooxygenase-2 (COX-2) mRNA by reverse transcription polymerase chain reaction (RT-PCR) technique and COX-2 protein expression using Western blot analysis. The results were analyzed using one-way analysis of variance (ANOVA). Tests of differences of the treatments were analyzed using the Tukey test and a value of P < .05 was considered statistically significant.
The paste and primer of the resin base adhesive and the liquid of glass ionomer adhesive showed decreasing survival rates after 24 hours of treatment (P < .05). All orthodontic adhesives induced COX-2 protein expression in human gingival fibroblasts. The exposure of quiescent human gingival fibroblasts to adhesives resulted in the induction of COX-2 mRNA expression. The investigations of the time-dependent COX-2 mRNA expression in adhesive-treated human gingival fibroblasts revealed different patterns.
The hypothesis is rejected. For orthodontic patients with gingival inflammation, except for those with oral hygiene problems, the activation of COX-2 expression by orthodontic adhesive may be one of the potential mechanisms.
检验树脂基和树脂混合玻璃离子基粘合剂在体外与原代人牙龈成纤维细胞接触后不会引起炎症的无效假设。
使用树脂基和树脂混合玻璃离子基粘合剂处理人牙龈成纤维细胞，采用MTT比色法评估细胞存活率，通过逆转录聚合酶链反应（RT-PCR）技术检测环氧合酶-2（COX-2）mRNA水平，并用蛋白质免疫印迹分析检测COX-2蛋白表达。结果采用单因素方差分析（ANOVA）进行分析。各处理组间差异的检验采用Tukey检验，P < 0.05的值被认为具有统计学意义。
树脂基粘合剂的糊剂和底漆以及玻璃离子粘合剂的液体在处理24小时后显示细胞存活率下降（P < 0.05）。所有正畸粘合剂均诱导人牙龈成纤维细胞中COX-2蛋白表达。静止的人牙龈成纤维细胞暴露于粘合剂后导致COX-2 mRNA表达的诱导。对粘合剂处理的人牙龈成纤维细胞中COX-2 mRNA表达的时间依赖性研究揭示了不同的模式。
该假设被拒绝。对于患有牙龈炎症的正畸患者，除了那些有口腔卫生问题的患者外，正畸粘合剂激活COX-2表达可能是潜在机制之一。
